Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Piumngam, P; Schachtrup, C; Owada, Y; Kondo, H; Promptmas, C; Spener, F.
Expression of liver-type fatty acid-binding protein in murine lung and its release into serum upon, challenge of lung with lipopolysaccharide
EUR J LIPID SCI TECHNOL. 2005; 107(3): 145-152.
Doi: 10.1002/ejlt.200501133
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- Führende Autor*innen der Med Uni Graz
- Spener Friedrich
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- Abstract:
- Fatty acid-binding proteins (FABP) in alveolar type II (TII) cells are required for surfactant synthesis and regulation. Beyond expression of heart-type (H-) and epidermal-type (E-) FABP in TII cells from mouse lung, we present the first evidence of the expression of liver-type (L-) FABP, by quantitative PCR and immunofluorescent confocal laser microscopy. Further, by making use of an acute mouse lung injury model, we examine whether these lipid-binding proteins are released into the bronchoalveolar fluid (BALF) and into the circulation upon challenge of the lung with lipopolysaccharide. Applying FABP-specific ELISAs, we found that neither H- nor E-FABP can be detected in BALF and serum above background levels, up to 24 h after insult. In contrast, L-FABP was detected in the BALF pellet, consisting of polymorphonuclear cells and alveolar macrophages, and in serum. A significant decrease in L-FABP levels in the BALF pellet was associated with a significant increase in serum levels 6 h post insult. As contributions of L-FABP from other organs were excluded, this protein could be used as a marker for acute lung injury.
- Find related publications in this database (Keywords)
- liver-type fatty acid-binding protein
- alveolar type II cell
- lipopolysaccaride
- acute lung damage
- serum