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Selected Publication:
Meyer, UJ; Trau, D; Key, G; Meusel, M; Spener, F.
Comparison of different types of immunoaffinity reactors in an electrochemical flow injection immunoanalysis system developed for residue analysis
BIOCATAL BIOTRANSFORM. 1999; 17(2): 103-124.
Doi: 10.3109/10242429909015226
Web of Science
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- Co-authors Med Uni Graz
- Spener Friedrich
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- Abstract:
- A comparison of different types of immunoreactors for a flow injection immunoanalysis (FIIA) system is presented. The use of the FIIA system was demonstrated here with the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). The hardware system centers around the immunoreactor. In the preferred reactor set-up, a column is filled with protein G-coated porous beads serving as the affinity matrix for the antibody. In the 2,4-D assay a 2,4-D-alkaline phosphatase conjugate competes with the analyte in the sample for binding to the anti-2,4-D antibody. Subsequently p-aminophenol, generated from the enzymatic reaction with p-aminophenyl phosphate, is detected at +/-150 mV at a carbon working electrode versus Ag/AgCl. The immunoreactor allows for more than 150 measurements. The fully automated assay procedure needs 15 min only for one measurement. For 2,4-D the working range for the determination in water samples was between 1 and 1000 mu g/L. Results obtained with the packed column reactor are compared to glassy capillary reactors and miniaturized silicon chip reactors used in previous studies.
- Find related publications in this database (Keywords)
- flow injection immunoanalysis
- 2,4-dichlorophenoxyacetic acid
- immunosensor
- biosensor
- amperometry
- pesticides