Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Buhlmann, C; Borchers, T; Pollak, M; Spener, F.
Fatty acid metabolism in human breast cancer cells (MCF7) transfected with heart-type fatty acid binding protein
MOL CELL BIOCHEM. MOLECULAR AND CELLULAR BIOCHEMISTRY; 199: 41-48.
Doi: 10.1023/A:1006986629206
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- Co-Autor*innen der Med Uni Graz
- Spener Friedrich
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- Abstract:
- The human breast cancer cell line MCF7 does not express heart-type fatty acid binding protein (H-FABP), a marker protein for differentiated mammary gland. MCF7 cells transfected with the bovine H-FABP cDNA expressed the corresponding protein and were characterized by growth inhibition and lower tumorgenicity in nude mice [22]. By enzyme linked immunoassay we now determined the amount of bovine H-FABP in these cells as 638 +/- 80 ng/mg protein and used the transfected cells to study the role of H-FABP in fatty acid metabolism. Compared to control cells the uptake of radioactively labelled palmitic acid and oleic acid into MCF7 cells after 30 or 60 min was increased by 67% in H-FABP expressing transfectants, demonstrating a stimulatory role for this FABP-type in fatty acid metabolism. However, preferential targeting of [14C]oleic acid into neutral or phospholipid classes was not observed by the criterion of high performance thin layer chromatography followed by autoradiography. A reason for the modest increase of fatty acid uptake in H-FABP transfected MCF7 cells may be the basal expression of epidermal-type FABP, which was detected for the first time in these cells. It appears that the small amount of E-FABP expressed in MCF7 cells fulfils the need of the cells for a cytosolic fatty acid carrier under culture conditions and that even high concentrations of another FABP do only slightly increase the uptake due to limitations of fatty acid transport through the plasma membrane or of metabolism.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Breast Neoplasms - metabolism
- Carbon Radioisotopes -
- Carrier Proteins - genetics Carrier Proteins - metabolism
- Cattle -
- Enzyme-Linked Immunosorbent Assay -
- Fatty Acid-Binding Proteins -
- Fatty Acids - metabolism
- Female -
- Humans -
- Myelin P2 Protein - genetics Myelin P2 Protein - metabolism
- Myocardium - metabolism
- Neoplasm Proteins -
- Oleic Acid - metabolism
- Palmitic Acid - metabolism
- Phosphatidylcholines - metabolism
- Recombinant Proteins - genetics Recombinant Proteins - metabolism
- Transfection -
- Tumor Cells, Cultured -
- Tumor Suppressor Proteins -
- Find related publications in this database (Keywords)
- fatty acid binding protein
- mammary derived growth inhibitor
- MCF7 cells
- fatty acid uptake
- transfected cells
- lipid analysis