Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
FELDBRUGGE, R; RENNEBERG, R; SPENER, F.
DEVELOPMENT AND PRACTICAL EVALUATION OF AN AMPEROMETRIC TRIGLYCERIDE SENSOR
SENSOR ACTUATOR B-CHEM. SENSORS AND ACTUATORS B-CHEMICAL; 19: 365-367.
Doi: 10.1016/0925-4005(93)00998-E
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- Co-Autor*innen der Med Uni Graz
- Spener Friedrich
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- Abstract:
- Quantitation of triglycerides is carried out in medical laboratories as well as in quality control departments in food, fat and oil industries. Our objective is to develop an amperometric triglyceride sensor with an enzymatic hydrolysis unit and a sensor unit with a thin polyurethane bienzyme membrane containing glycerol kinase (EC 2.7.1.30) and glycerol-3-phosphate oxidase (EC 1.1.3.21), sandwiched between two dialysis membranes and mounted onto a platinum electrode in a flow-through cell combined with pumps, autosampler and potentiostat. The complete system contains a computer-controlled data acquisition and analyzing unit. Complete hydrolysis of triglyceride samples is attained within 15 min with the aid of a previously freeze-dried mix containing triacylglycerol acylhydrolase (lipase EC 3.1.1.3), carboxylic-ester hydrolase (esterase EC 3.1.1.1), detergent, ATP and buffer. 25 samples per hour can be analyzed with a linear sensor signal from 5 to 1000 mumol/l and a standard deviation of 2% (repetitive measurements).