Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SCHOEMAKER, M; SPENER, F.
ENZYMATIC FLOW-INJECTION ANALYSIS FOR ESSENTIAL FATTY-ACIDS
SENSOR ACTUATOR B-CHEM. SENSORS AND ACTUATORS B-CHEMICAL; 19: 607-609.
Doi: 10.1016/0925-4005(93)01100-I
Web of Science
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- Co-Autor*innen der Med Uni Graz
- Spener Friedrich
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- Abstract:
- A flow-injection-analysis system has been developed for the determination of essential fatty acids. The enzyme lipoxygenase (EC 1.13.11.12) was immobilized on CNBr-activated Sepharose to form an enzyme column. The oxygen consumption owing to the lipoxygenase-catalyzed conversion from polyunsaturated fatty acid to hydroperoxy fatty acid was monitored by a flow-through cell containing a Clark-type oxygen electrode. Calibration curves for linoleic and alpha-linolenic acid were nearly identical. A linear correlation was observed between current decrease and fatty acid concentration from 0.1 to 1.2 mM.