Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SCHUCH, R; BRUMMEL, M; SPENER, F.
BETA-KETOACYL-ACYL CARRIER PROTEIN (ACP) SYNTHASE-III IN CUPHEA-LANCEOLATA SEEDS - IDENTIFICATION AND ANALYSIS OF REACTION-PRODUCTS
J PLANT PHYSIOL. JOURNAL OF PLANT PHYSIOLOGY; 143: 556-560.
Doi: 10.1016/S0176-1617(11)81823-7
Web of Science
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- Co-Autor*innen der Med Uni Graz
- Spener Friedrich
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- Abstract:
- The activities of enzymes of the fatty acid synthase (FAS) were investigated in the seeds of Cuphea lanceolata, an annual crop producing high levels of decanoic acid, by monitoring the incorporation of [1-C-14]acetate from [1-C-14]acetyl-CoA into acyl-acyl carrier protein (ACP). The influence of cerulenin, an inhibitor of beta-ketoacyl-acyl carrier protein synthases (KAS, EC. 2.3.1.41.) I and II, was also examined. The activities of the FAS in absence and presence of 100 mu M cerulenin were almost identical, indicating that the FAS in C. lanceolata seeds contains the cerulenin insensitive KAS III, which uses acetyl-CoA as substrate. The products of the FAS reaction of C. lanceolata, acyl-ACPs, were separated by urea-PAGE and analyzed by electroblot and autoradiography. Product acyl-ACPs were identified both by comparing their migration behaviors in urea-PAGE with standards synthesized from C-14-fatty acids and E. coli ACP and by reversed phase HPLC of fatty acids obtained after chemical hydrolysis. The major product of the KAS III reaction was butyryl-ACP, whereas hexanoyl- and octanoyl-ACPs were produced in small proportions. In absence of cerulenin octanoyl- and decanoyl-ACPs were the major products.
- Find related publications in this database (Keywords)
- CUPHEA LANCEOLATA
- ACETYL-COA, ACP TRANSACYLASE
- ACYL CARRIER PROTEIN
- FATTY ACID SYNTHASE
- BETA-KETOACYL-ACP SYNTHASE
- SEED