Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Naseri, JI; Truong, NT; Hörentrup, J; Kuballa, P; Vogel, A; Rompel, A; Spener, F; Krebs, B.
Porcine purple acid phosphatase: heterologous expression, characterization, and proteolytic analysis.
Arch Biochem Biophys. 2004; 432(1):25-36
Doi: 10.1016/j.abb.2004.08.008
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Spener Friedrich
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- Abstract:
- Uteroferrin is an iron-binding glycoprotein, which is abundantly synthesized in porcine uterine glandular endometrium and believed to be involved in maternal/fetal iron transport. In the present study, uteroferrin has been cloned and functionally expressed using baculovirus-infected insect host cells Spodoptera frugiperda. The work also addresses the possible role of proteolytic cleavage to facilitate the release of uteroferrin-bound iron. The enzyme secreted in culture medium exhibits a molecular mass and catalytic properties similar to native porcine uteroferrin. The specific activity was estimated at 233 U/mg using p-nitrophenyl phosphate as substrate. Partial cleavage of the enzyme with trypsin resulted in a 1.7-fold enhancement in specific activity and a two-subunit polypeptide as observed in preparations of most mammalian purple acid phosphatases. Digestion with the aspartic protease pepsin resulted in a 2.5-fold enzyme inactivation correlated with the appearance of low molecular weight polypeptide fragments and the release of enzyme-bound iron.
- Find related publications in this database (using NLM MeSH Indexing)
- Acid Phosphatase - chemistry
- Amino Acid Sequence -
- Animals -
- Baculoviridae - genetics
- Base Sequence -
- Blotting, Western -
- Cloning, Molecular -
- DNA, Complementary - metabolism
- DNA, Viral - metabolism
- Dose-Response Relationship, Drug -
- Electrophoresis, Polyacrylamide Gel -
- Escherichia coli - metabolism
- Genetic Vectors -
- Glycoproteins - chemistry
- Glycoside Hydrolases - pharmacology
- Humans -
- Immunoblotting -
- Insects -
- Iron - chemistry Iron - metabolism
- Isoenzymes -
- Metalloproteins - genetics Metalloproteins - metabolism
- Mice -
- Molecular Sequence Data -
- Peptides - chemistry
- RNA - metabolism
- Rats -
- Recombinant Proteins - chemistry
- Reverse Transcriptase Polymerase Chain Reaction -
- Spectrophotometry -
- Spodoptera -
- Swine -
- Time Factors -
- Transfection -
- Ultraviolet Rays -
- Find related publications in this database (Keywords)
- uteroferrin
- purple acid phosphatase
- porcine iron transport
- proteolysis
- metalloenzyme
- baculovirus