Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Puskás, LG; Bereczki, E; Sántha, M; Vigh, L; Csanádi, G; Spener, F; Ferdinandy, P; Onochy, A; Kitajka, K.
Cholesterol and cholesterol plus DHA diet-induced gene expression and fatty acid changes in mouse eye and brain.
Biochimie. 2004; 86(11):817-824
Doi: 10.1016/j.biochi.2004.10.004
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Co-Autor*innen der Med Uni Graz
- Spener Friedrich
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Both cholesterol and polyunsaturated fatty acid (PUFA) metabolism play an important role in retinal and brain development and function. Dietary intake of cholesterol is accompanied with higher risk of heart disease and was suggested to have a role in the pathogenesis of Alzheimer's disease, while dietary PUFAs were reported to act in an opposite way. The same phenomena could be seen in case of inflammation. These effects are mainly realized through gene expression changes. In the present study, the effects of dietary cholesterol and the combination of cholesterol and fish oil were analyzed on the modulation of fatty acid composition and gene expression in the brain and in the eye. At the transcription level, specific changes could be detected in both tissues among transcription factor genes coding for sterol regulatory element binding proteins, retinoid X receptors and peroxisome proliferator-activated receptors, and different fatty acid binding protein genes by using quantitative real-time PCR. In the eye, cholesterol diet attenuated the positive effects of fish oil on inflammatory gene expression as the combined diet resulted in increased RNAm level of phospholipase A-2, inducible nitric oxide synthase, TNF-alpha, COX-1, COX-2 and cytokine, ICAM-1. This induction was absent in the brain. Complex changes could be also recorded in the fatty acid composition of lipids extracted from eye and brain tissue due to the dietary intervention. One of the most interesting changes was the reduced level of docosahexaenoic acid by cholesterol in the eye. Our results on fatty acid composition and gene expression changes may open up new alleys in understanding the complex roles of cholesterol and PUFAs in normal and pathological visual and brain function.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Brain - drug effects Brain - metabolism
- Carrier Proteins - genetics Carrier Proteins - metabolism
- Cholesterol, Dietary - administration and dosage Cholesterol, Dietary - metabolism
- Diet -
- Docosahexaenoic Acids - administration and dosage Docosahexaenoic Acids - metabolism
- Eye - drug effects Eye - metabolism
- Fatty Acid-Binding Proteins -
- Fatty Acids - metabolism
- Gene Expression Regulation - drug effects Gene Expression Regulation - physiology
- Inflammation -
- Male -
- Mice -
- Mice, Inbred C57BL -
- Mice, Inbred CBA -
- Molecular Sequence Data -
- Transcription Factors - genetics Transcription Factors - metabolism
- Transcription, Genetic -
- Find related publications in this database (Keywords)
- gene expression
- cholesterol
- docosahexaenoic acid
- polyunsaturated fatty acids
- real-time PCR
- brain
- eye