Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

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SHR Neuro Krebs Kardio Lipid Stoffw Microb

Pelsers, MM; Hanhoff, T; Van der Voort, D; Arts, B; Peters, M; Ponds, R; Honig, A; Rudzinski, W; Spener, F; de Kruijk, JR; Twijnstra, A; Hermens, WT; Menheere, PP; Glatz, JF.
Brain- and heart-type fatty acid-binding proteins in the brain: tissue distribution and clinical utility.
Clin Chem. 2004; 50(9):1568-1575 Doi: 10.1373/clinchem.2003.030361 [OPEN ACCESS]
Web of Science PubMed FullText FullText_MUG

Co-Autor*innen der Med Uni Graz: Spener Friedrich
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Abstract:: BACKGROUND: Detection of brain injury by serum markers is not a standard procedure in clinical practice, although several proteins, such as S100B, neuron-specific enolase (NSE), myelin basic protein, and glial fibrillary acidic protein, show promising results. We investigated the tissue distribution of brain- and heart-type fatty acid-binding proteins (B-FABP and H-FABP) in segments of the human brain and the potential of either protein to serve as plasma marker for diagnosis of brain injury. METHODS: B-FABP and H-FABP were measured immunochemically in autopsy samples of the brain (n = 6) and in serum samples from (a) patients with mild traumatic brain injury (MTBI; n = 130) and (b) depressed patients undergoing bilateral electroconvulsive therapy (ECT; n = 14). The protein markers S100B and NSE were measured for comparison. Reference values of B-FABP and H-FABP were established in healthy individuals (n = 92). RESULTS: The frontal, temporal, and occipital lobes, the striatum, the pons, and the cerebellum had different tissue concentrations of B-FABP and of H-FABP. B-FABP ranged from 0.8 microg/g wet weight in striatum tissue to 3.1 microg/g in frontal lobe. H-FABP was markedly higher, ranging from 16.2 microg/g wet weight in cerebellum tissue to 39.5 microg/g in pons. No B-FABP was detected in serum from healthy donors. H-FABP serum reference value was 6 microg/L. In the MTBI study, serum B-FABP was increased in 68% and H-FABP in 70% of patients compared with S100B (increased in 45%) and NSE (increased in 51% of patients). In ECT, serum B-FABP was increased in 6% of all samples (2 of 14 patients), whereas H-FABP was above its upper reference limit (6 microg/L) in 17% of all samples (8 of 14 patients), and S100B was above its upper reference limit (0.3 microg/L) in 0.4% of all samples. CONCLUSIONS: B-FABP and H-FABP patterns differ among brain tissues, with the highest concentrations in the frontal lobe and pons, respectively. However, in each part of the brain, the H-FABP concentration was at least 10 times higher than that of B-FABP. Patient studies indicate that B-FABP and H-FABP are more sensitive markers for minor brain injury than the currently used markers S100B and NSE.
Find related publications in this database (using NLM MeSH Indexing): Adult -; Aged -; Biological Markers - blood; Blotting, Western -; Brain - metabolism; Brain Injuries - blood Brain Injuries - diagnosis Brain Injuries - metabolism; Carrier Proteins - blood Carrier Proteins - metabolism; Electroconvulsive Therapy -; Fatty Acid-Binding Proteins -; Female -; Humans -; Immunoassay -; Male -; Middle Aged -; Phosphopyruvate Hydratase - blood; Reference Values -; S100 Proteins - blood; Statistics, Nonparametric -; Tissue Distribution -