Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Adida, A; Spener, F.
Adipocyte-type fatty acid-binding protein as inter-compartmental shuttle for peroxisome proliferator activated receptor gamma agonists in cultured cell.
Biochim Biophys Acta. 2006; 1761(2):172-181
Doi: 10.1016/j.bbalip.2006.02.006
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- Führende Autor*innen der Med Uni Graz
- Spener Friedrich
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- Abstract:
- We and others showed earlier that liver-type, epidermal-type and adipocyte-type (A-) fatty acid-binding proteins (FABPs) mediate peroxisome proliferator activated receptor (PPAR) dependent gene expression by channelling their ligand (fatty acid or drug) to the nuclear receptors via direct protein/protein interaction. To clarify mechanistic details of this signaling path, we address here A-FABP import into the nucleus and its interaction with PPARgamma. Making use of COS cells transfected with wild-type or mutant A-FABPs, we exclude posttranslational modification of A-FABP as import signal and provide evidence for both, ligand-dependent and ligand-independent nuclear translocation. With the aid of in vitro pull down assay we demonstrate that specific interaction of A-FABP with PPARgamma isoforms does not require ligand. Moreover, A-FABP binds not only to the ligand-binding domain including hinge domain (domains DEF), but also to the DNA-binding domain including AB domains (domains ABC) of PPARgamma.
- Find related publications in this database (using NLM MeSH Indexing)
- Active Transport, Cell Nucleus -
- Animals -
- Base Sequence -
- Biological Transport, Active -
- COS Cells -
- Cell Compartmentation -
- Cercopithecus aethiops -
- DNA, Complementary - genetics
- Fatty Acid-Binding Proteins - genetics Fatty Acid-Binding Proteins - metabolism
- Ligands -
- Mice -
- Mutagenesis, Site-Directed -
- PPAR alpha - metabolism
- PPAR gamma - agonists PPAR gamma - chemistry PPAR gamma - metabolism
- PPAR-beta - metabolism
- Protein Processing, Post-Translational -
- Protein Structure, Tertiary -
- Recombinant Proteins - genetics Recombinant Proteins - metabolism
- Find related publications in this database (Keywords)
- fatty acid-binding protein
- peroxisome proliferator activated receptor
- fatty acid signaling
- ligand dependence
- subcellular localization
- protein/protein interaction