Medizinische Universität Graz - Research portal
Selected Publication:
Schütt, BS; Abbadi, A; Loddenkötter, B; Brummel, M; Spener, F.
Beta-ketoacyl-acyl carrier protein synthase IV: a key enzyme for regulation of medium-chain fatty acid synthesis in Cuphea lanceolata seeds.
Planta. 2002; 215(5):847-854
Doi: 10.1007/s00425-002-0803-8
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- Leading authors Med Uni Graz
- Spener Friedrich
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- Abstract:
- With the aim of elucidating the mechanisms involved in the biosynthesis of medium-chain fatty acids in Cuphea lanceolata Ait., a crop accumulating up to 90% decanoic acid in seed triacylglycerols, cDNA clones of a beta-ketoacyl-acyl carrier protein (ACP) synthase IV (clKAS IV, EC 2.3.1.41) were isolated from C. lanceolata seed embryos. The amino acid sequence deduced from clKAS IV cDNA showed 80% identity to other plant KAS II-type enzymes, 55% identity towards plant KAS I and over 90% towards other Cuphea KAS IV-type sequences. Recombinant clKAS IV was functionally overexpressed in Escherichia coli, and substrate specificity of purified enzyme showed strong preference for elongation of short-chain and medium-chain acyl-ACPs (C4- to C10-ACP) with nearly equal activity. Further elongation steps were catalysed with distinctly less activity. Moreover, short- and medium-chain acyl-ACPs exerted a chain-length-specific and concentration-dependent substrate inhibition of clKAS IV. Based on these findings a regulatory mechanism for medium-chain fatty acid synthesis in C. lanceolata is presented.
- Find related publications in this database (using NLM MeSH Indexing)
- 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase - genetics 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase - isolation and purification 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase - metabolism
- Cloning, Molecular -
- Cuphea - enzymology Cuphea - genetics
- DNA, Complementary - chemistry DNA, Complementary - genetics
- Escherichia coli - genetics
- Fatty Acids - biosynthesis
- Gene Expression Regulation, Enzymologic -
- Gene Expression Regulation, Plant -
- Molecular Sequence Data -
- Seeds - enzymology Seeds - genetics
- Sequence Analysis, DNA -
- Substrate Specificity -
- Find related publications in this database (Keywords)
- condensing enzyme
- Cuphea (fatty acids)
- fatty acid metabolism
- medium-chain fatty acid
- thioesterase