Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Vollenbroeker, B; Fobker, M; Specht, B; Bartetzko, N; Erren, M; Spener, F; Hohage, H.
Receptor assay based on surface plasmon resonance for the assessment of the complex formation activity of cyclosporin A and its metabolites.
Int J Clin Pharmacol Ther. 2003; 41(6):248-260
Doi: 10.5414/CPP41248
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- Co-Autor*innen der Med Uni Graz
- Spener Friedrich
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- Abstract:
- OBJECTIVE: A new automated receptor assay has been used to determine the complex formation activity of cyclosporin A (CsA) and its metabolites in whole blood. METHODS: CsA in vivo forms a complex with cyclophilin A and calcineurin leading to an inhibition of the calmodulin-dependent phosphatase activity of calcineurin. The equilibrium complex formation gives information about the potential immunosuppressive activity of CsA and its metabolites. To measure the amount of this complex the authors developed an automated receptor assay based on an optical biosensor (Biacore) with surface plasmon resonance (SPR) technology. RESULTS: In the range of 50-300 nM CsA, the intra-day coefficient of variation (CV) was 7.2%, and the inter-day CV was 10.1%. Measuring range of the assay was 10-500 nM with a detection limit of 5 nM and a processing time of 10 min. Recovery rate for sample pretreatment was 74 +/- 5%. 193 blood specimens from heart transplant recipients were analyzed with 3 different methods. The results determined with the receptor assay were correlated with those obtained by fluorescence polarization immunoassay (FPIA; r = 0.599) and high-performance liquid chromatography (HPLC; r = 0.615). CONCLUSION: The receptor assay determines the complex formation activity of CsA and its metabolites with high sensitivity and precision.
- Find related publications in this database (using NLM MeSH Indexing)
- Chromatography, High Pressure Liquid -
- Cyclophilin A - metabolism
- Cyclosporine - analysis Cyclosporine - blood Cyclosporine - metabolism
- Drug Monitoring - methods
- Fluorescence Polarization Immunoassay -
- Gas Chromatography-Mass Spectrometry -
- Humans -
- Immunosuppressive Agents - analysis Immunosuppressive Agents - blood Immunosuppressive Agents - metabolism
- Phosphoric Monoester Hydrolases - metabolism
- Protein Binding -
- Sensitivity and Specificity -
- Surface Plasmon Resonance - methods
- Find related publications in this database (Keywords)
- cyclosporin A
- complex formation activity
- surface plasmon resonance
- receptor assay