Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Schreiber, A; Specht, B; Pelsers, MM; Glatz, JF; Börchers, T; Spener, F.
Recombinant human heart-type fatty acid-binding protein as standard in immunochemical assays.
Clin Chem Lab Med. 1998; 36(5):283-288
Doi: 10.1515/CCLM.1998.048
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- Co-Autor*innen der Med Uni Graz
- Spener Friedrich
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- Abstract:
- Cytoplasmic heart-type fatty acid-binding protein has recently gained much attention in clinical diagnosis as a very early marker of acute myocardial infarction. Immunoassays have been developed for determination of this protein in plasma and urine samples. In the present study it is shown that those types of fatty acid-binding proteins which are abundant in tissues other than heart and muscle do not interfere with immunochemical determination of heart-type fatty acid-binding protein. To provide sufficient protein of consistent quality as standard in these immunoassays, human heart-type fatty acid-binding protein was cloned, expressed in Escherichia coli and purified to homogeneity. For quantitation of the recombinant protein its extinction coefficient was determined. Comparison of the recombinant and tissue-derived proteins by a variety of methods revealed both proteins to show similar kinetic as well as equilibrium constants with respect to two monoclonal antibodies currently applied in immunochemical detection of heart-type fatty acid-binding protein. Both preparations were indistiguishable in sandwich-ELISA and immunosensor measurements. A high stability of the recombinant protein was proven by ELISA measurements during storage and several freeze and thaw cycles. Thus, recombinant and tissue-derived heart-type fatty acid-binding proteins are immunochemically equivalent. The recombinant human heart-type fatty acid-binding protein is now available as standard for immunoassays.
- Find related publications in this database (using NLM MeSH Indexing)
- Antibodies, Monoclonal -
- Antigen-Antibody Reactions -
- Carrier Proteins - analysis Carrier Proteins - genetics Carrier Proteins - standards
- Cloning, Molecular -
- Cross Reactions -
- Drug Stability -
- Enzyme-Linked Immunosorbent Assay - standards
- Escherichia coli - genetics
- Fatty Acid-Binding Proteins -
- Fatty Acids - metabolism
- Humans -
- Immunochemistry - standards
- Kinetics -
- Myelin P2 Protein - analysis Myelin P2 Protein - genetics Myelin P2 Protein - standards
- Myocardial Infarction - diagnosis Myocardial Infarction - metabolism
- Myocardium - metabolism
- Neoplasm Proteins -
- Recombinant Proteins - analysis Recombinant Proteins - genetics Recombinant Proteins - standards
- Reference Standards -
- Tumor Suppressor Proteins -
- Find related publications in this database (Keywords)
- heart-type fatty acid-binding protein (H-FABP)
- recombinant protein
- myocardial infarction
- BIAcore
- biosensor