Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Guthmann, F; Hohoff, C; Fechner, H; Humbert, P; Börchers, T; Spener, F; Rüstow, B.
Expression of fatty-acid-binding proteins in cells involved in lung-specific lipid metabolism.
Eur J Biochem. 1998; 253(2):430-436
Doi: 10.1046/j.1432-1327.1998.2530430.x
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- Co-Autor*innen der Med Uni Graz
- Spener Friedrich
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- Abstract:
- Members of the fatty-acid-binding protein (FABP) family are thought to play an important role in fatty acid transport within the cytosol and thus to be involved in lipid metabolism. As previous data on the occurrence of distinct FABP types in total lung are contradictory, we determined the expression of FABP types in three isolated cell types of rat lung, which are characterised by active lipid metabolism. Alveolar type-II cells synthesise, store and secrete pulmonary surfactant, a phospholipid-rich surface-tension-lowering agent, whereas lung fibroblasts, localised adjacent to the alveolar type-II cells, are assumed to provide neutral lipid substrate to alveolar type-II cells around birth, and alveolar macrophages are known to degrade complex lipids. Initial screening by reverse transcriptase PCR revealed the occurrence of heart (H-), epidermal (E-) and liver FABP in rat lung, the latter being not detectable in the three cell types studied. Cells were analysed by northern and western blotting, then quantitatively by sandwich ELISA, for which recombinant rat E-FABP was prepared. E-FABP mRNA was found in all three cell types, and E-FABP was detected in the following amounts: 240.9 +/- 19.0 ng/mg cytosolic protein in alveolar type-II cells; 172.3 +/- 0.7 ng/mg protein for lung fibroblasts; and 36.9 +/- 3.5 ng/mg protein for alveolar macrophages. This indicates a basic function of E-FABP in cellular lipid metabolism. In contrast, H-FABP probably is involved in the metabolism of neutral lipids because H-FABP mRNA was found only in lung fibroblasts with a corresponding protein level of 315.5 +/- 6.9 ng/mg. Small amounts of H-FABP protein were present in alveolar type-II cells and alveolar macrophages.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Carrier Proteins - genetics Carrier Proteins - metabolism
- Enzyme-Linked Immunosorbent Assay -
- Fatty Acid-Binding Proteins -
- Liver - metabolism
- Lung - metabolism
- Macrophages - metabolism
- Male -
- Myelin P2 Protein - genetics Myelin P2 Protein - metabolism
- Neoplasm Proteins -
- Nerve Tissue Proteins -
- Polymerase Chain Reaction -
- Rats -
- Rats, Wistar -
- Skin - metabolism
- Find related publications in this database (Keywords)
- fatty-acid-binding protein
- alveolar type-II cell
- lung fibroblast
- alveolar macrophage
- ELISA