Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Potthoff, AP; Haalck, L; Spener, F.
Inhibition of lipases from Chromobacterium viscosum and Rhizopus oryzae by tetrahydrolipstatin.
Biochim Biophys Acta. 1998; 1389(2):123-131
Doi: 10.1016/S0005-2760(97)00160-4
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- Führende Autor*innen der Med Uni Graz
- Spener Friedrich
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- Abstract:
- Tetrahydrolipstatin is known as an inhibitor for pancreatic lipase but not for microbial lipases. In this paper we demonstrate that in the presence of water-insoluble substrates like tributyrin or olive oil, tetrahydrolipstatin inhibits the lipases of Chromobacterium viscosum and Rhizopus oryzae, although with different potency. In contrast to porcine pancreatic lipase, which forms an irreversible and covalent enzyme-inhibitor complex with tetrahydrolipstatin, the inhibition of the microbial lipases is reversible as the inhibitor can be removed from the enzyme-inhibitor complex by solvent extraction. Moreover, after inhibition of Chromobacterium viscosum lipase tetrahydrolipstatin remains chemically unchanged.
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- Find related publications in this database (Keywords)
- microbial lipase
- pancreatic lipase
- tetrahydrolipstatin
- non-covalent inhibition
- competitive type inhibition
- (Chromobacterium viscosum)
- (Rhizopus oryzae)