Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Gerdes, M; Meusel, M; Spener, F.
Development of a displacement immunoassay by exploiting cross-reactivity of a monoclonal antibody.
Anal Biochem. 1997; 252(1):198-204
Doi: 10.1006/abio.1997.2301
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- Co-Autor*innen der Med Uni Graz
- Spener Friedrich
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- Abstract:
- An enzyme-linked immunosorbent assay-based displacement assay was developed for the determination of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). Advantage was taken of the cross-reactivity of a monoclonal anti-2,4-D antibody toward 2-methyl-4-chlorophenoxyacetic acid (MCPA). MCPA was conjugated with bovine serum albumin (BSA), immobilized on the surface of a microtiter plate, and saturated with the anti-2,4-D antibody. Due to the low affinity of the antibody toward MCPA (cross-reactivity of approximately 30%), the addition of 2,4-D resulted in a displacement of the antibody. Remaining antibodies were subsequently detected using a peroxidase-labeled goat anti-mouse antibody. The detection limit was as low as 0.1 microgram/liter for 2,4-D, which complies with the European Union Drinking Water Directives. When 2,4-D-BSA was used instead of MCPA-BSA conjugates, no significant displacement of bound antibody was observed.
- Find related publications in this database (using NLM MeSH Indexing)
- 2,4-Dichlorophenoxyacetic Acid - analysis 2,4-Dichlorophenoxyacetic Acid - immunology
- 2-Methyl-4-chlorophenoxyacetic Acid - analysis 2-Methyl-4-chlorophenoxyacetic Acid - chemistry 2-Methyl-4-chlorophenoxyacetic Acid - immunology
- Animals -
- Antibodies, Monoclonal - immunology Antibodies, Monoclonal - isolation and purification
- Cross Reactions -
- Enzyme-Linked Immunosorbent Assay - methods
- Haptens - analysis Haptens - immunology
- Indoleacetic Acids - chemistry
- Serum Albumin, Bovine - chemistry
- Find related publications in this database (Keywords)
- 2,4-dichlorophenoxyacetic acid (2,4-D)
- 2-methyl-4-chlorophenoxyacetic acid (MCPA)
- herbicide
- ELISA
- displacement assay
- monoclonal antibodies
- pesticides