Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Trau, D; Theueri, T; Wilmer, M; Meusel, M; Spener, F.
Development of an amperometric flow injection immunoanalysis system for the determination of the herbicide 2,4-dichlorophenoxyacetic acid in water.
Biosens Bioelectron. 1997; 12(6):499-510
Doi: 10.1016/S0956-5663(97)00007-9
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- Co-Autor*innen der Med Uni Graz
- Spener Friedrich
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- Abstract:
- An amperometric flow injection immunoanalysis (FIIA) system based on an immunoreactor with immobilized biocomponents on a silica surface has been developed for the determination of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). In the antigen coating mode the hapten was immobilized and monoclonal primary antibody against 2,4-D together with alkaline phosphatase (AP)-labelled secondary antibody were used as sensing elements in a titration assay. In the antibody coating mode a biotinylated monoclonal antibody was immobilized on the surface of the immunoreactor and a 2,4-D-AP-conjugate was used for detection. For electrochemical measurements p-aminophenol enzymatically generated from p-aminophenyl phosphate was oxidized at a carbon working electrode at +150 mV versus Ag/AgCl. The system enabled the determination of 2,4-D in drinking water samples in the range from 0.2 to 70 micrograms/l. The whole system was computer controlled with a measuring time of 12 min for one determination.
- Find related publications in this database (using NLM MeSH Indexing)
- 2,4-Dichlorophenoxyacetic Acid - analysis
- Alkaline Phosphatase -
- Animals -
- Antibodies, Monoclonal - diagnostic use
- Dose-Response Relationship, Immunologic -
- Electrochemistry -
- Herbicides - analysis
- Hydrogen-Ion Concentration -
- Immunoassay - instrumentation
- Immunoenzyme Techniques -
- Mice -
- Water - analysis
- Water Pollutants - analysis
- Find related publications in this database (Keywords)
- flow injection immunoanalysis
- FIIA
- biosensor
- immunosensor
- amperometry
- herbicides
- phenoxycarbonic acids
- 2,4-D