Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Wendzinski, F; Gründig, B; Renneberg, R; Spener, F.
Highly sensitive determination of hydrogen peroxide and peroxidase with tetrathiafulvalene-based electrodes and the application in immunosensing.
Biosens Bioelectron. 1997; 12(1):43-52
Doi: 10.1016/0956-5663(96)89088-9
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Co-Autor*innen der Med Uni Graz
- Spener Friedrich
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Redox mediators enable an efficient electron transfer between redox enzymes and the electrochemical surface of amperometric sensors. A stable and highly sensitive signal was obtained using a tetrathiafulvalene (TTF)-modified graphite electrode. In the presence of horseradish peroxidase (HRP), hydrogen peroxide (H2O2) was monitored with a detection limit of 7 nM at a potential of +20 mV versus a saturated calomel electrode (SCE). With a constant concentration of H2O2, the detection limit for HRP was found to be 150 pM. The accuracy of consecutive measurement of HRP in flow-through systems was improved by short-time polarizing the TTF-modified graphite electrode at +100 mV versus Ag/AgCl/3 M KCl. Using the TTF-modified graphite electrode in an immuno-sandwich approach, rabbit-immunoglobulin G was monitored in the range 5-100 ng/ml.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Biosensing Techniques -
- Horseradish Peroxidase - analysis
- Hydrogen Peroxide - analysis
- Hydrogen-Ion Concentration -
- Rabbits -
- Temperature -
- Find related publications in this database (Keywords)
- amperometric immunosensor
- graphite electrode
- flow-through system
- horseradish peroxidase
- hydrogen peroxide
- mediator electrode
- tetrathiafulvalene