Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Myers-Payne, SC; Hubbell, T; Pu, L; Schnütgen, F; Börchers, T; Wood, WG; Spener, F; Schroeder, F.
Isolation and characterization of two fatty acid binding proteins from mouse brain.
J Neurochem. 1996; 66(4):1648-1656
Doi: 10.1046/j.1471-4159.1996.66041648.x
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Co-Autor*innen der Med Uni Graz
- Spener Friedrich
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Two fatty acid binding proteins (FABPs) were isolated from Swiss Webster mouse brains. Neither protein cross-reacted with antisera to recombinant liver L-FABP. One protein, designated brain H-FABP, migrated on tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as a single band at 14.5 kDa with pl 4.9. Brain H-FABP bound NBD-stearic acid and cis-parinaric acid with K D values near 0.02 and 0.5 microM, respectively. Brain H-FABP cross-reacted with affinity-purified antisera to recombinant heart H-FABP. The second protein, mouse brain B-FABP, migrated on tricine SDS-PAGE gels as a doublet at 16.0 and 15.5 kDa with pl values of 4.5 and 4.7, respectively. Brain B-FABP bound NBD-stearic and cis-parinaric acid with K D values near 0.01 and 0.7 microM, respectively. The brain B-FABP doublet was immunoreactive with affinity-purified antibodies against recombinant mouse brain B-FABP, but not with affinity-purified antibodies against heart H-FABP. (3H)Oleate competition binding indicated that the two brain FABPs had distinct ligand binding specificities. Both bound fatty acids, fatty acyl CoA, and lysophosphatidic acid. Although both preferentially bound unsaturated fatty acids, twofold differences in specific saturated fatty acid binding were observed. Brain B-FABP and brain H-FABP represented 0.1% and 0.01% of brain total cytosolic protein, respectively. In summary, mouse brain contains two native fatty acid binding proteins, brain H-FABP and brain B-FABP.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Binding, Competitive -
- Brain Chemistry -
- Carrier Proteins - chemistry Carrier Proteins - isolation and purification Carrier Proteins - metabolism
- Chromatography, DEAE-Cellulose -
- Chromatography, High Pressure Liquid -
- Electrophoresis, Polyacrylamide Gel -
- Fatty Acid-Binding Proteins -
- Fatty Acids - metabolism
- Isoelectric Focusing -
- Mice -
- Myelin P2 Protein - chemistry Myelin P2 Protein - isolation and purification Myelin P2 Protein - metabolism
- Neoplasm Proteins -
- Nerve Tissue Proteins -
- Protein Binding -
- Find related publications in this database (Keywords)
- brain
- fatty acid binding protein
- fatty acid
- mouse