Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Eggenstein, C; Borchardt, M; Diekmann, C; Gründig, B; Dumschat, C; Cammann, K; Knoll, M; Spener, F.
A disposable biosensor for urea determination in blood based on an ammonium-sensitive transducer.
Biosens Bioelectron. 1999; 14(1):33-41
Doi: 10.1016/S0956-5663(98)00103-1
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- Führende Autor*innen der Med Uni Graz
- Spener Friedrich
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- Abstract:
- A potentiometric urea-sensitive biosensor using a NH4(+)-sensitive disposable electrode in double matrix membrane (DMM) technology as transducer is described. The ion-sensitive polymer matrix membrane was formed in the presence of an additional electrochemical inert filter paper matrix to improve the reproducibility in sensor production. The electrodes were prepared from one-side silver-coated filter paper, which is encapsulated for insulation by a heat-sealing film. A defined volume of the NH4(+)-sensitive polymer matrix membrane cocktail was deposited on this filter paper. To obtain the urea-biosensor a layer of urease was cast onto the ion-sensitive membrane. Poly (carbamoylsulfonate) hydrogel, produced from a hydrophilic polyurethane prepolymer blocked with bisulfite, served as immobilisation material. The disposable urea sensitive electrode was combined with a disposable Ag/AgCl reference electrode to obtain the disposable urea biosensor. The sensor responded rapidly and in a stable manner to changes in urea concentrations between 7.2 x 10(-5) and 2.1 x 10(-2)mol/l. The detection limit was 2 x 10(-5) mol/l urea and the slope in the linear range 52 mV/decade. By taking into consideration the influence of the interfering K(+)- and Na(+)-ions the sensor can be used for the determination of urea in human blood and serum samples (diluted or undiluted). A good correlation was found with the data obtained by the spectrophotometric routine method.
- Find related publications in this database (using NLM MeSH Indexing)
- Biosensing Techniques -
- Humans -
- Membranes, Artificial -
- Potentiometry -
- Quaternary Ammonium Compounds -
- Transducers -
- Urea - blood
- Find related publications in this database (Keywords)
- disposable biosensor
- disposable reference electrode
- potentiometric biosensor
- urea biosensor
- blood urea