Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Bleck, B; Hohoff, C; Binas, B; Rüstow, B; Dixkens, C; Hameister, H; Börchers, T; Spener, F.
Cloning and chromosomal localisation of the murine epidermal-type fatty acid binding protein gene (Fabpe).
Gene. 1998; 215(1):123-130
Doi: 10.1016/S0378-1119(98)00262-5
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Führende Autor*innen der Med Uni Graz
- Spener Friedrich
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- We succeeded in cloning the gene encoding the murine epidermal-type fatty acid binding protein (E-FABP). To avoid the screening of pseudogenes, the presence of which was shown by PCR, we designed an intron-specific probe and screened a bacterial artificial chromosome library from mouse embryonic stem cells. One of the clones obtained was analysed by restriction with various enzymes and an 11-kb EcoRI fragment with the complete gene was subcloned. The gene revealed the canonical exon/intron FABP structure consisting of four exons (112, 173, 102 and 544bp, respectively) and three introns (2217, 327 and 546bp, respectively). The exon sequences were identical with the cDNA encoding mouse E-FABP (Krieg, P., Feil, S., Fürstenberger, G., Bowden, T.G., 1993. Tumor-specific overexpression of a novel keratinocyte lipid-binding protein. Identification and characterisation of a cloned sequence activated during multistage carcinogenesis in mouse skin. J. Biol. Chem. 268, 17362-17369). Of the 5' region, 2470bp were sequenced and searched for transcription factor binding sites. Putative responsive elements within the promoter region were identified that may be responsible for the wide expression observed for E-FABP in mouse tissues. The 11-kb EcoRI fragment was used to localise Fabpe on chromosome 3 in the region 3A1-3 by fluorescence in-situ hybridisation.
- Find related publications in this database (using NLM MeSH Indexing)
- Amino Acid Sequence -
- Animals -
- Base Sequence -
- Blotting, Western -
- Carrier Proteins - analysis Carrier Proteins - genetics
- Chromosome Mapping -
- Chromosomes - genetics
- Cloning, Molecular -
- DNA - chemistry DNA - genetics
- Epidermis - chemistry
- Fatty Acid-Binding Proteins -
- Genes - genetics
- Mice -
- Molecular Sequence Data -
- Myelin P2 Protein - analysis Myelin P2 Protein - genetics
- Neoplasm Proteins -
- Nerve Tissue Proteins -
- Restriction Mapping -
- Sequence Analysis, DNA -
- Sequence Homology, Amino Acid -
- Tissue Distribution -
- Find related publications in this database (Keywords)
- fluorescence in-situ hybridisation
- gene cloning
- gene map
- tissue expression
- transcription factor binding sites