Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Guthmann, F; Haupt, R; Looman, AC; Spener, F; Rüstow, B.
Fatty acid translocase/CD36 mediates the uptake of palmitate by type II pneumocytes.
Am J Physiol. 1999; 277(1 Pt 1):L191-L196
Doi: 10.1152/ajplung.1999.277.1.L191
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- Co-Autor*innen der Med Uni Graz
- Spener Friedrich
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- Abstract:
- Type II pneumocytes, which synthesize, store, and secrete pulmonary surfactant, require exogenous fatty acids, in particular palmitic acid, for maximum surfactant synthesis. The uptake of palmitate by type II pneumocytes is thought to be protein mediated, but the protein involved has not been characterized. Here we show by RT-PCR and Northern blot analysis that rat type II pneumocytes express the mRNA for fatty acid translocase (FAT/CD36), a membrane-associated protein that is known to facilitate the uptake of fatty acids into adipocytes. The deduced amino acid sequence from rat type II pneumocytes reveals 98% identity to the FAT/CD36 sequence obtained from rat adipocytes. The uptake of palmitate by type II pneumocytes follows Michaelis-Menten kinetics (Michaelis-Menten constant = 11.9 +/- 1.8 nM; maximum velocity = 62.7 +/- 5.8 pmol. min(-1). 5 x 10(5) pneumocytes(-1)) and decreases reversibly under conditions of ATP depletion to 35% of control uptake. Incubation of cells at 0 degrees C inhibited the uptake of palmitate almost completely, whereas depletion of potassium was without effect. Preincubation of the cells with bromobimane or phloretin decreases the uptake of palmitate significantly as does preincubation with sulfo-N-succinimidyl oleate, the specific inhibitor of FAT/CD36 (C. M. Harmon, P. Luce, A. H. Beth, and N. A. Abumrad. J. Membr. Biol. 121: 261-268, 1991). From these data, we conclude that FAT/CD36 is expressed in type II pneumocytes and mediates the uptake of palmitate in a saturable and energy-dependent manner. The data suggest that the uptake process is independent of the formation of coated pits and endocytotic vesicles.
- Find related publications in this database (using NLM MeSH Indexing)
- Amino Acid Sequence - genetics
- Animals -
- Antigens, CD36 -
- Bicyclo Compounds - pharmacology
- Drug Synergism -
- Lung - cytology Lung - metabolism
- Membrane Glycoproteins - antagonists and inhibitors Membrane Glycoproteins - genetics Membrane Glycoproteins - physiology
- Oleic Acids - pharmacology
- Organic Anion Transporters -
- Palmitic Acid - antagonists and inhibitors Palmitic Acid - pharmacokinetics
- Phloretin - pharmacology
- Rats -
- Rats, Wistar -
- Succinimides - pharmacology
- Find related publications in this database (Keywords)
- fatty acid uptake
- uptake kinetics
- lung