Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Gerdes, M; Meusel, M; Spener, F.
Influence of antibody valency in a displacement immunoassay for the quantitation of 2,4-dichlorophenoxyacetic acid.
J Immunol Methods. 1999; 223(2):217-226
Doi: 10.1016/S0022-1759(98)00211-7
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- Co-Autor*innen der Med Uni Graz
- Spener Friedrich
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- Abstract:
- The influence of antibody valency in a displacement immunoassay was investigated by comparing the whole antibody molecule with the corresponding Fab-fragment. The displacement immunoassay for the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) takes advantage of the cross-reactivity of monoclonal anti-2,4-D antibodies and the Fab-fragments toward immobilized 2-methyl-4-chlorophenoxyacetic acid (MCPA). Due to the low affinity of the antibodies toward MCPA (cross-reactivity of approximately 30%), the addition of 2,4-D resulted in a displacement of the antibodies or the fragments. The detection limits obtained with whole anti-2,4-D antibodies and Fab-fragments were 0.1 microg/l and 0.01 microg/l 2,4-D, respectively. The whole antibodies and the Fab-fragments show similarities, such as the cross-reactivity toward MCPA (26% and 33%), and some characteristics of the calibration curve, for example the large detection range and the sensitivity. In contrast to the bivalent antibodies, however, increasing the hapten/protein ratios of the immobilized MCPA-BSA conjugates did not affect the detection limit when using the Fab-fragments. Moreover, kinetic experiments reveal a faster displacement reaction with the Fab-fragments. A disadvantage of using the Fab-fragments is the generation of lower absorbance values in the ELISA.
- Find related publications in this database (using NLM MeSH Indexing)
- 2,4-Dichlorophenoxyacetic Acid - immunology 2,4-Dichlorophenoxyacetic Acid - metabolism
- Antibodies - chemistry Antibodies - immunology Antibodies - metabolism
- Antibody Affinity -
- Binding, Competitive - immunology
- Calibration -
- Cross Reactions -
- Haptens - metabolism
- Immunoassay - methods
- Immunoglobulin Fab Fragments - immunology Immunoglobulin Fab Fragments - metabolism
- Kinetics -
- Find related publications in this database (Keywords)
- antibody valency
- fab-fragment
- 2,4-dichlorophenoxyacetic acid (2,4-D)
- 2-methyl-4-chlorophenoxyacetic acid (MCPA)
- displacement assay
- rapid assay