Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Wolfrum, C; Buhlmann, C; Rolf, B; Börchers, T; Spener, F.
Variation of liver-type fatty acid binding protein content in the human hepatoma cell line HepG2 by peroxisome proliferators and antisense RNA affects the rate of fatty acid uptake.
Biochim Biophys Acta. 1999; 1437(2):194-201
Doi: 10.1016/S1388-1981(99)00008-6
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Führende Autor*innen der Med Uni Graz
- Spener Friedrich
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- The liver-type fatty acid binding protein (L-FABP), a member of a family of mostly cytosolic 14-15 kDa proteins known to bind fatty acids in vitro and in vivo, is discussed to play a role in fatty acid uptake. Cells of the hepatoma HepG2 cell line endogenously express this protein to approximately 0.2% of cytosolic proteins and served as a model to study the effect of L-FABP on fatty acid uptake, by manipulating L-FABP expression in two approaches. First, L-FABP content was more than doubled upon treating the cells with the potent peroxisome proliferators bezafibrate and Wy14,643 and incubation of these cells with [1-14C]oleic acid led to an increase in fatty acid uptake rate from 0.55 to 0.74 and 0.98 nmol/min per mg protein, respectively. In the second approach L-FABP expression was reduced by stable transfection with antisense L-FABP mRNA yielding seven clones with L-FABP contents ranging from 0.03% to 0.14% of cytosolic proteins. This reduction to one sixth of normal L-FABP content reduced the rate of [1-14C]oleic acid uptake from 0.55 to 0. 19 nmol/min per mg protein, i.e., by 66%. The analysis of peroxisome proliferator-treated cells and L-FABP mRNA antisense clones revealed a direct correlation between L-FABP content and fatty acid uptake.
- Find related publications in this database (using NLM MeSH Indexing)
- Bezafibrate - pharmacology
- Carcinoma, Hepatocellular -
- Carrier Proteins - biosynthesis Carrier Proteins - genetics
- Fatty Acid-Binding Proteins -
- Fatty Acids - metabolism
- Humans -
- Myelin P2 Protein - biosynthesis Myelin P2 Protein - genetics
- Neoplasm Proteins -
- Peroxisome Proliferators - pharmacology
- Pyrimidines - pharmacology
- RNA, Antisense -
- Transfection -
- Tumor Cells, Cultured -
- Tumor Suppressor Proteins -
- Find related publications in this database (Keywords)
- liver-type fatty acid binding protein
- HepG2 cell
- bezafibrate
- Wy14,643
- antisense RNA
- fatty acid uptake