Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Lang, D; Haalck, L; Hofmann, B; Hecht, HJ; Spener, F; Schmid, RD; Schomburg, D.
Crystallization and preliminary X-ray analysis of a lipase from Chromobacterium viscosum.
Acta Crystallogr D Biol Crystallogr. 1994; 50(Pt 2):225-227
Doi: 10.1107/S0907444993009941
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- Spener Friedrich
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- Abstract:
- Lipase from Chromobacterium viscosum has been purified to homogeneity and crystallized in a form suitable for X-ray diffraction analysis from 10-14% polyethylene glycol 4000 and 10-14% 2-methyl-2,4-pentane diol at pH 6.4 in the presence of 0.25%(w/v) n-octyl-beta-D-glucopyranoside. These crystals belong to space group P2(1)2(1)2 with refined lattice constants a = 41.1 A, b= 156.8, c = 43.6 A, indicating a cell content of one monomer per asymmetric unit of the crystal. The crystals diffract to a resolution of 2.2 A.