Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Müller-Fahrnow, A; Egner, U; Jones, TA; Rüdel, H; Spener, F; Saenger, W.
Three-dimensional structure of fatty-acid-binding protein from bovine heart.
Eur J Biochem. 1991; 199(2):271-276
Doi: 10.1111/j.1432-1033.1991.tb16120.x
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- Co-Autor*innen der Med Uni Graz
- Spener Friedrich
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- Abstract:
- The complex of fatty-acid-binding protein (FABP) from bovine heart (cFABP, pI4.9) with endogenous lipid was crystallized in the presence of ammonium sulfate as precipitant. The needle-shaped crystals belong to the monoclinic space group C2, with unit-cell constants a = 5.262(6) nm, b = 7.631(8) nm, c = 3.945(5) nm and beta = 94.47(9) degrees. A native data set to 0.35 nm resolution was collected using synchrotron radiation and film methods. An initial model for the three-dimensional structure of the protein was constructed based on the crystal structure of the related bovine P2 myelin protein [Jones, T.A., Bergfors, T., Sedzik, J. & Unge, T. (1988) EMBO J. 7, 1597-1604] to which the amino acid sequence of bovine cFABP was adapted. Energy minimizations were carried out under different conditions using both an all-atom and a united-atom force field. The optimized models were used to determine the crystal structure of cFABP by molecular-replacement techniques. The structure was refined by simulated annealing to R = 0.267. As the bound lipid is heterogeneous, it could not be located in the electron-density map and/or the attained resolution was not sufficient. Bovine cFABP is composed of ten antiparallel beta strands forming a beta barrel, and by two alpha helices. The structural features are similar to those of other members of the superfamily of hydrophobic molecule transporters.
- Find related publications in this database (using NLM MeSH Indexing)
- Amino Acid Sequence -
- Animals -
- Carrier Proteins - chemistry Carrier Proteins - isolation and purification
- Cattle -
- Fatty Acid-Binding Proteins -
- Fatty Acids - metabolism
- Models, Molecular -
- Molecular Sequence Data -
- Myocardium - metabolism
- Neoplasm Proteins -
- Protein Conformation -
- Sequence Homology, Nucleic Acid -
- X-Ray Diffraction -