Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Unterberg, C; Börchers, T; Højrup, P; Roepstorff, P; Knudsen, J; Spener, F.
Cardiac fatty acid-binding proteins. Isolation and characterization of the mitochondrial fatty acid-binding protein and its structural relationship with the cytosolic isoforms.
J Biol Chem. 1990; 265(27):16255-16261
Doi: 10.1016/S0021-9258(17)46216-8
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- Co-Autor*innen der Med Uni Graz
- Spener Friedrich
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- Abstract:
- In the course of our studies on the structural diversity of the isoforms of cardiac fatty acid-binding proteins (cFABPs), a cardiac-type FABP from the matrix of bovine heart mitochondria was purified to homogeneity and obtained as a single 15-kDa protein with an isoelectric point of 4.9. The primary structures of this protein and of the two isoforms isolated from the cytosol (pI4.9-cFABP and pI 5.1-cFABP) were investigated by means of plasma desorption mass spectrometry and sequencing of peptides. All three proteins are amino-terminally blocked with an acetyl group and shown to be colinear with the sequence deduced from a cDNA clone for bovine heart fatty acid-binding protein (Billich, S., Wissel, T., Kratzin, H., Hahn, U., Hagenhoff, B., Lezius, A. G., and Spener, F. (1988) Eur. J. Biochem. 175, 549-556) except for the residue at position 98. This residue is demonstrated to be the molecular origin of bovine cFABP isoforms since pI 5.1-cFABP contains Asn98 in accordance with the sequence derived from the cDNA, whereas in pI 4.9-cFABP, this position is occupied by Asp98. Moreover, mitochondrial FABP is identical to pI 4.9-cFABP. Molecular masses of pI 4.9-cFABP (14,679 +/- 10 Da) and pI 5.1-cFABP (14,678 +/- 20 Da) determined by plasma desorption mass spectrometry coincide with that calculated from the cDNA (14,673 Da). Hence, residues linked to these proteins by posttranslational modification are not present, and the Asn-Asp exchange is the sole origin of heterogeneity of mitochondrial and cytosolic fatty acid-binding proteins from bovine heart.
- Find related publications in this database (using NLM MeSH Indexing)
- Amino Acid Sequence -
- Animals -
- Carrier Proteins - isolation and purification Carrier Proteins - metabolism
- Cattle -
- Chromatography, Gel -
- Chromatography, High Pressure Liquid -
- Chromatography, Ion Exchange -
- Cytosol - metabolism
- Fatty Acid-Binding Proteins -
- Fatty Acids - metabolism
- Mass Spectrometry -
- Mitochondria, Heart - metabolism
- Molecular Sequence Data -
- Myocardium - metabolism
- Neoplasm Proteins -
- Peptide Fragments - isolation and purification
- Peptide Mapping -
- Sequence Homology, Nucleic Acid -