Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Meusel, M; Renneberg, R; Spener, F; Schmitz, G.
Development of a heterogeneous amperometric immunosensor for the determination of apolipoprotein E in serum.
Biosens Bioelectron. 1995; 10(6-7):577-586
Doi: 10.1016/0956-5663(95)96933-P
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Spener Friedrich
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- Abstract:
- Apolipoprotein (apo) E is a constituent of serum lipoproteins and can serve as a diagnostic parameter for the assessment of disorders of lipid metabolism. For the determination of apo E in serum a sandwich-type amperometric immunosensor using disposable membranes was developed. The best results were obtained by using a site-directed attachment of a monoclonal capture antibody to a hydrazide-functionalized membrane surface. Bound antigen was then determined with the aid of a polyclonal antibody labelled with alkaline phosphatase in conjunction with p-aminophenyl phosphate as substrate. In this approach a carbon working electrode (vs. Hg/HgCl2) was used, and enzymatically generated p-aminophenol could be monitored with a detection limit of 40 pmol and a linear range of 26-20000 nM. The sensor displayed a linear response from 50 to 1000 ng ml-1 apo E. In contrast, antibody coupling through primary amino groups led to a total loss of antigen binding capacity in this assay configuration. The approach with site-directed immobilization however, not only allowed the determination of apo E in serum, but also the determination of antibody cross-reactivity against the apolipoproteins AI, AII and B.
- Find related publications in this database (using NLM MeSH Indexing)
- Aminophenols - analysis
- Apolipoproteins E - blood
- Biosensing Techniques -
- Cross Reactions -
- Electrochemistry -
- Humans -
- Reproducibility of Results -
- Find related publications in this database (Keywords)
- ALKALINE PHOSPHATASE
- AMPEROMETRIC IMMUNOSENSOR
- APOLIPOPROTEIN E
- CARBON ELECTRODE
- GLUCOSE OXIDASE
- P-AMINOPHENYL PHOSPHATE
- SITE-DIRECTED IMMOBILIZATION