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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Ikeoka, DT; Pachler, C; Mader, JK; Bock, G; Neves, AL; Svehlikova, E; Feichtner, F; Koehler, G; Wrighton, CJ; Pieber, TR; Ellmerer, M.
Lipid-heparin infusion suppresses the IL-10 response to trauma in subcutaneous adipose tissue in humans.
Obesity (Silver Spring). 2011; 19(4):715-721
Doi: 10.1038/oby.2010.227
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- Leading authors Med Uni Graz
- Ikeoka Dimas
- Co-authors Med Uni Graz
- Ellmerer Martin
- Feichtner Franz
- Köhler Gerd
- Mader Julia
- Pachler Christoph
- Pieber Thomas
- Svehlikova Eva
- Treiber Gerlies
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- Abstract:
- An imbalance between pro- and anti-inflammatory cytokine productions in adipose tissue is thought to contribute to chronic, systemic, low-grade inflammation and consequently to an increased risk of cardiovascular complications in obese and type 2 diabetic patients. Nonesterified fatty acids (NEFA), whose serum levels are elevated in such patients, have been shown to interfere with cytokine production in vitro. In order to evaluate the effects of elevated NEFA levels on cytokine production in adipose tissue in vivo we used an 18-gauge open-flow microperfusion (OFM) catheter to induce local inflammation in the subcutaneous adipose tissue (SAT) of healthy volunteers and to sample interstitial fluid (IF) specifically from the inflamed tissue. In two crossover studies, nine subjects received either an intravenous lipid-heparin infusion to elevate circulating NEFA levels or saline over a period of 28 h. The former increased the circulating levels of triglycerides (TGs), NEFA, glucose, and insulin over the study period. NEFA effects on locally induced inflammation were estimated by measuring the levels of a panel adipokines in the OFM probe effluent. Interleukin-6 (IL-6), IL-8, tumor necrosis factor-α (TNF-α) and monocyte chemoattractant protein-1 (MCP-1) levels increased during the study period but were not affected by lipid-heparin infusion. In contrast, the level of IL-10, an anti-inflammatory cytokine, was significantly reduced during the final hour of lipid-heparin infusion (saline: 449.2 ± 105.9 vs. lipid-heparin: 65.4 ± 15.4 pg/ml; P = 0.02). These data provide the first in vivo evidence that elevated NEFA can modulate cytokine production by adipose tissue.
- Find related publications in this database (using NLM MeSH Indexing)
- Adipokines - metabolism
- Adipokines - secretion
- Adult -
- Blood Glucose - analysis
- Catheters -
- Chemokine CCL2 - blood
- Cross-Over Studies -
- Cytokines - biosynthesis
- Diabetes Mellitus, Type 2 - complications
- Diabetes Mellitus, Type 2 - immunology
- Fatty Acids, Nonesterified - administration & dosage
- Fatty Acids, Nonesterified - blood
- Heparin - metabolism
- Humans -
- Inflammation - pathology
- Insulin - blood
- Interleukin-10 - metabolism
- Interleukin-6 - blood
- Interleukin-8 - blood
- Lipids - blood
- Male -
- Obesity - complications
- Obesity - immunology
- Retrospective Studies -
- Subcutaneous Fat - metabolism
- Subcutaneous Fat - physiopathology
- Triglycerides - blood
- Tumor Necrosis Factor-alpha - blood
- Young Adult -