Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Etchart, N; Desmoulins, PO; Chemin, K; Maliszewski, C; Dubois, B; Wild, F; Kaiserlian, D.
Dendritic cells recruitment and in vivo priming of CD8+ CTL induced by a single topical or transepithelial immunization via the buccal mucosa with measles virus nucleoprotein.
J Immunol. 2001; 167(1):384-391
Doi: 10.4049/jimmunol.167.1.384
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- Abstract:
- The buccal mucosa, a prototype of pluristratified mucosal epithelia, contains a network of directly accessible class II(+) epithelial dendritic cells (DC), similar to skin Langerhans cells. We showed that a single buccal immunization with measles virus nucleoprotein (NP), by either topical application onto or intradermal injection in the buccal mucosa, induced in vivo priming of protective class I-restricted specific CD8(+) CTL. Both routes of immunization with NP induced a rapid recruitment of DC into the mucosa, which peaked at 2 h and decreased by 24 h. Treatment of mice with Flt3 ligand resulted in an increased number of DC in the buccal mucosa and enhanced the frequency of IFN-gamma-producing NP-specific effectors and the NP-specific CTL response generated after buccal immunization with NP. Finally, NP-pulsed bone marrow-derived DC induced NP-specific IFN-gamma-producing cells upon adoptive transfer to naive mice. These data demonstrate that a viral protein delivered to DC of the buccal mucosa induces in vivo priming of protective anti-viral CD8(+) CTL.
- Find related publications in this database (using NLM MeSH Indexing)
- Adjuvants, Immunologic - administration and dosage
- Administration, Buccal -
- Administration, Cutaneous -
- Animals -
- Antigen Presentation -
- Bone Marrow Cells - immunology Bone Marrow Cells - virology
- CD4-Positive T-Lymphocytes - immunology
- Cell Division - immunology
- Cell Movement - immunology
- Dendritic Cells - cytology Dendritic Cells - immunology Dendritic Cells - transplantation Dendritic Cells - virology
- Distemper - mortality Distemper - prevention and control
- Distemper Virus, Canine - immunology
- Dose-Response Relationship, Immunologic -
- Epitopes, T-Lymphocyte - immunology
- Female -
- Injections, Intradermal -
- Lymphocyte Activation - immunology
- Measles Vaccine - administration and dosage Measles Vaccine - immunology
- Membrane Proteins - administration and dosage
- Mice -
- Mice, Inbred BALB C -
- Mouth Mucosa - cytology Mouth Mucosa - immunology Mouth Mucosa - virology
- Nucleoproteins - administration and dosage Nucleoproteins - genetics Nucleoproteins - immunology
- T-Lymphocytes, Cytotoxic - immunology T-Lymphocytes, Cytotoxic - virology
- Vaccines, Synthetic - administration and dosage Vaccines, Synthetic - immunology
- Viral Proteins - administration and dosage Viral Proteins - genetics Viral Proteins - immunology