Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Desvignes, C; Estèves, F; Etchart, N; Bella, C; Czerkinsky, C; Kaiserlian, D.
The murine buccal mucosa is an inductive site for priming class I-restricted CD8+ effector T cells in vivo.
Clin Exp Immunol. 1998; 113(3):386-393
Doi: 10.1046/j.1365-2249.1998.00671.x
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- Co-Autor*innen der Med Uni Graz
- Liechtenstein Nathalie
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- Abstract:
- The present study shows that Langerhans cells of the buccal mucosa and the skin share a similar phenotype, including in situ expression of MHC class II, the mannose receptor DEC-205 and CD11c, and absence of the costimulatory molecules B7.1, B7.2 and CD40 as well as Fas. Application of 2,4-dinitrofluorobenzene (DNFB) onto the buccal mucosa is associated with a rapid migration of dendritic cells (DC) to the epithelium and induction of B7.2 expression on some DC. Buccal sensitization with DNFB elicited a specific contact sensitivity (CS) in response to skin challenge, mediated by class I-restricted CD8+ effector T cells and down-regulated by class II-restricted CD4+ T cells, demonstrated by the lack of priming of class I-deficient mice and the enhanced response of class II-deficient mice, respectively. CS induced by buccal immunization is associated with priming of class I-restricted CD8+ effector T cells endowed with hapten-specific cytotoxic activity. Thus, the buccal epithelium is an inductive site, equivalent to the epidermis, for the generation of CS independent of CD4 help, and of cytotoxic T lymphocyte (CTL) responses mediated by class I-restricted CD8+ T cells. We propose that immunization through the buccal mucosa, which allows antigen presentation by epithelial DC efficient for priming systemic class I-restricted CD8+ CTL, may be a valuable approach for single-dose mucosal vaccination with subunit vaccines.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Antigens, CD80 - analysis
- CD8-Positive T-Lymphocytes - immunology
- Cheek -
- Dendritic Cells - physiology
- Dermatitis, Contact - etiology
- Dinitrofluorobenzene -
- Female -
- Histocompatibility Antigens Class I - physiology
- Immunization -
- Langerhans Cells - immunology
- Lymphocyte Activation -
- Mice -
- Mice, Inbred BALB C -
- Mice, Inbred C57BL -
- Mouth Mucosa - immunology
- Rats -
- Find related publications in this database (Keywords)
- epithelial dendritic cells
- buccal mucosa
- CD8 T cells
- contact sensitivity