Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Etchart, N; Wild, F; Kaiserlian, D.
Mucosal and systemic immune responses to measles virus haemagglutinin in mice immunized with a recombinant vaccinia virus.
J Gen Virol. 1996; 77 ( Pt 10)(3):2471-2478
Doi: 10.1099/0022-1317-77-10-2471
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- Liechtenstein Nathalie
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- Abstract:
- The immune response to a vaccinia virus recombinant expressing the measles virus haemagglutinin (VV-HA) was compared after parenteral or mucosal immunizations in mice. Parenteral immunizations with 10(6) p.f.u. VV-HA induced HA-specific antibody-producing cells (IgG>IgA) and HA-specific class I-restricted cytotoxic T lymphocytes (CTL) in the spleen. In contrast, intranasal administrations of 10(6) p.f.u. of VV-HA induced HA-specific spot-forming cells in the spleen (IgG>IgA) and the lungs (IgA>IgG), and HA-specific CTL in the spleen. Co-immunization by the nasal route with VV-HA and cholera toxin enhanced HA-specific immune responses. Oral immunizations with 10(8) p.f.u. of VV-HA generated low numbers of HA-specific IgA-producing cells in the lamina propria of the gut, and a weak HA-specific CTL activity in the spleen and mesenteric lymph nodes. Oral co-immunization with VV-HA and cholera toxin greatly enhanced the level of HA-specific spot-forming cells in the lamina propria (IgA>IgG). Interestingly, intrajejunal immunizations with 10(8) p.f.u. VV-HA alone induced high levels of anti-HA IgG-producing cells in the spleen and anti-HA IgA-secreting cells in the lamina propria of the gut. These data show that (i) VV-HA by the nasal route is immunogenic and generates a measles-specific mucosal immune response in the lung, which represents the primary site of replication of measles virus and that (ii) VV-HA can also induce measles-specific immunity in the intestine provided that it is protected from degradation in the gastrointestinal tract, or that cholera toxin is used as an adjuvant.
- Find related publications in this database (using NLM MeSH Indexing)
- Administration, Intranasal -
- Administration, Oral -
- Animals -
- Antibodies, Viral -
- Cercopithecus aethiops -
- Cholera Toxin - immunology
- Female -
- Genetic Vectors -
- Hela Cells -
- Hemagglutinins, Viral - immunology
- Humans -
- Immunity, Mucosal - immunology
- Immunoglobulin A -
- Intestinal Mucosa - immunology
- Intestines -
- Lung - immunology
- Measles virus - immunology
- Mice -
- Mice, Inbred BALB C -
- Recombination, Genetic -
- Spleen - cytology Spleen - immunology
- T-Lymphocytes, Cytotoxic -
- Vaccinia virus - genetics
- Vero Cells -