Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Sollner, S; Schober, M; Wagner, A; Prem, A; Lorkova, L; Palfey, BA; Groll, M; Macheroux, P.
Quinone reductase acts as a redox switch of the 20S yeast proteasome.
EMBO Rep. 2009; 10(1): 65-70.
Doi: 10.1038/embor.2008.218
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- Co-Autor*innen der Med Uni Graz
- Wagner Andrea
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- Abstract:
- The proteasome has an essential function in the intracellular degradation of protein in eukaryotic cells. We found that the dimeric quinone reductase Lot6 uses the flavin mononucleotide (FMN)-binding site to bind to the 20S proteasome with a 1:2 stoichiometry-that is, one 20S proteasome molecule can associate with two quinone reductases. Furthermore, reduction of the FMN cofactor by either NADH or light irradiation results in the binding of the b-Zip transcription factor Yap4 to the Lot6-proteasome complex, indicating that recruitment of the transcription factor depends on the redox state of the quinone reductase. Here, we show that binding of Yap4 to the complex not only protects it from ubiquitin-independent proteasomal degradation, but also regulates its cellular localization. In non-stressed wild-type cells, we did not detect any Yap4 in the nucleus, whereas Yap4 was present in the nuclei from quinone-stressed yeast cultures. Thus, the Lot6-proteasome complex can be regarded as a redox switch in which the quinone reductase acts as a sensor for oxidative stress.
- Find related publications in this database (using NLM MeSH Indexing)
- FMN Reductase - genetics FMN Reductase - metabolism
- Flavins - metabolism
- NAD(P)H Dehydrogenase (Quinone) - genetics NAD(P)H Dehydrogenase (Quinone) - metabolism
- Nuclear Proteins - genetics Nuclear Proteins - metabolism
- Oxidation-Reduction -
- Proteasome Endopeptidase Complex - metabolism
- Protein Binding -
- Saccharomyces cerevisiae - enzymology
- Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism
- Transcription Factors - genetics Transcription Factors - metabolism
- Find related publications in this database (Keywords)
- Lot6
- redox state
- 20S proteasome
- Yap4
- transcription factor