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Selected Publication:
Riedmann, B; Maier, H; Weiss, H; Schwab, G; Labeck, B; Totsch, M; Schmid, KW; Ofner, D.
Silver stained nucleolar organizer regions associated protein (AgNOR) analysis: A major prognostic factor in gastric carcinoma
TUMORDIAGN THER. 1998; 19(2): 35-41.
Web of Science
- Co-authors Med Uni Graz
- Tötsch Martin
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- Abstract:
- Background: Argyrophilic nucleolar organizer regions associated proteins (AgNORs) are known to reflect cellular transcriptional activity. Their standardized visualization at light microscopical level with subsequent morphometric analysis have been shown to provide a simple, inexpensive and reliable routine parameter to determine proliferative activity, which is considered to be of significance regarding prognosis in cancer patients. Patients and Method: Quantitation of AgNORs was carried out by standardized staining and morphometric analysis on a series of 110 surgical resected gastric carcinomas. The AgNOR content per nuclei is expressed by standardized AgNOR parameters (coefficient of variation (CV) of number and area of AgNORs per nucleus). Results: Standardized AgNOR parameters were shown to be statistically significantly (CV of AgNOR number: p<0.001; CV of AgNOR area: p<0.01) correlated with the clinical course of patients after surgical resection of gastric carcinoma. A multivariate analysis of all clinico-pathological features showed that R stage (p = 0.0001), UICC stage (p = 0.0001), and AgNOR quantity (p = 0.01) were statistically significantly and independently associated with survival. Conclusions: Standardized analysis of AgNORs is proposed as an important independent prognostic factor in surgical resected gastric carcinoma. The method is suitable for routine use on paraffin embedded archival material.
- Find related publications in this database (Keywords)
- morphometric AgNOR analysis
- gastric carcinoma
- tumor stage
- prognosis