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Akinremi, TO; Nazeer, S; Tötsch, M.
Reduced alcohol use in the staining of Pap smears: a satisfactory low-cost protocol for cervical cancer screening.
Acta Cytol. 2005; 49(2):169-172
Doi: 10.1159/000326127
Web of Science
PubMed
FullText
FullText_MUG
- Co-authors Med Uni Graz
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Tötsch Martin
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- Abstract:
- OBJECTIVE: To describe a low-cost Papanicolaou staining procedure that can be applied to conventional and monolayer gynecologic preparations. STUDY DESIGN: The amount of alcohol consumed in the procedure, which normally accounts for > 80% of the cost of processing, was reduced drastically by (1) using only 1 modified cytoplasmic counterstain (EA type), thereby (2) reducing the number of alcohol rinses by over half Orange-G dye is omitted. RESULTS: The resultant effect of the modified staining protocol is quite satisfactory and attractive to screening eyes: nuclear details are sharp and crisp, while the cytoplasm contains transparent differential staining with blue-green and pink. CONCLUSION: A reduction in the cost of staining should encourage cervical cancer screening, especially in developing countries, where cost is a limiting factor, thus making it possible for more women to be screened without increasing the cost of the program.
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Azo Compounds -
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Coloring Agents - economics
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Cost-Benefit Analysis -
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Ethanol - economics
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Female -
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Humans -
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Mass Screening - economics Mass Screening - methods Mass Screening - standards
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Methanol - economics
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Nigeria -
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Staining and Labeling - economics Staining and Labeling - methods Staining and Labeling - standards
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Uterine Cervical Neoplasms - diagnosis
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Vaginal Smears - economics Vaginal Smears - methods Vaginal Smears - standards
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Papanicolaou smear
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alcohols
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staining
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mass screening
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cervical neoplasms