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Akinremi, TO; Nazeer, S; Tötsch, M.
Reduced alcohol use in the staining of Pap smears: a satisfactory low-cost protocol for cervical cancer screening.
Acta Cytol. 2005; 49(2):169-172 Doi: 10.1159/000326127
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Co-Autor*innen der Med Uni Graz
Tötsch Martin
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Abstract:
OBJECTIVE: To describe a low-cost Papanicolaou staining procedure that can be applied to conventional and monolayer gynecologic preparations. STUDY DESIGN: The amount of alcohol consumed in the procedure, which normally accounts for > 80% of the cost of processing, was reduced drastically by (1) using only 1 modified cytoplasmic counterstain (EA type), thereby (2) reducing the number of alcohol rinses by over half Orange-G dye is omitted. RESULTS: The resultant effect of the modified staining protocol is quite satisfactory and attractive to screening eyes: nuclear details are sharp and crisp, while the cytoplasm contains transparent differential staining with blue-green and pink. CONCLUSION: A reduction in the cost of staining should encourage cervical cancer screening, especially in developing countries, where cost is a limiting factor, thus making it possible for more women to be screened without increasing the cost of the program.
Find related publications in this database (using NLM MeSH Indexing)
Azo Compounds -
Coloring Agents - economics
Cost-Benefit Analysis -
Ethanol - economics
Female -
Humans -
Mass Screening - economics Mass Screening - methods Mass Screening - standards
Methanol - economics
Nigeria -
Staining and Labeling - economics Staining and Labeling - methods Staining and Labeling - standards
Uterine Cervical Neoplasms - diagnosis
Vaginal Smears - economics Vaginal Smears - methods Vaginal Smears - standards

Find related publications in this database (Keywords)
Papanicolaou smear
alcohols
staining
mass screening
cervical neoplasms
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