Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Elia, N; Tapponnier, M; Matthay, MA; Hamacher, J; Pache, JC; Brundler, MA; Totsch, M; De Baetselier, P; Fransen, L; Fukuda, N; Morel, DR; Lucas, R.
Functional identification of the alveolar edema reabsorption activity of murine tumor necrosis factor-alpha.
Am J Respir Crit Care Med. 2003; 168(9):1043-1050
Doi: 10.1164/rccm.200206-618OC
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Tötsch Martin
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Tumor necrosis factor-alpha (TNF-alpha) activates sodium channels in Type II alveolar epithelial cells, an important mechanism for the reported fluid resorption capacity of the cytokine. Both TNF-alpha receptor-dependent and -independent effects were proposed for this activity in vitro, the latter mechanism mediated by the lectin-like domain of the molecule. In this study, the relative contribution of the receptor-dependent versus receptor-independent activities was investigated in an in situ mouse lung model and an ex vivo rat lung model. Fluid resorption due to murine TNF-alpha (mTNF-alpha) was functional in mice that were genetically deficient in both types of mTNF-alpha receptor, establishing the importance of mTNF-alpha receptor-independent effects in this species. In addition, we assessed the capacity of an mTNF-alpha-derived peptide (mLtip), which activates sodium transport by a receptor-independent mechanism, to reduce lung water content in an isolated, ventilated, autologous blood-perfused rat lung model. The results show that in this model, mLtip, in contrast to mTNF-alpha, produced a progressive recovery of dynamic lung compliance and airway resistance after alveolar flooding. There was also a significant reduction in lung water. These results indicate that the receptor-independent lectin-like domain of mTNF-alpha has a potential physiological role in the resolution of alveolar edema in rats and mice.
- Find related publications in this database (using NLM MeSH Indexing)
- Amiloride - pharmacology
- Animals -
- Biological Transport - physiology
- Diuretics - pharmacology
- Female -
- Lung - pathology
- Male -
- Mice -
- Mice, Inbred C57BL -
- Models, Animal -
- Organ Size -
- Peptide Fragments - metabolism
- Pulmonary Edema - metabolism
- Rats -
- Rats, Sprague-Dawley -
- Receptors, Tumor Necrosis Factor - deficiency Receptors, Tumor Necrosis Factor - metabolism
- Respiratory Mechanics - drug effects
- Sodium Channels - drug effects Sodium Channels - metabolism
- Tumor Necrosis Factor-alpha - metabolism
- Find related publications in this database (Keywords)
- edema
- cytokine
- sodium transport