Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Landgraeber, S; von Knoch, M; Löer, F; Brankamp, J; Tsokos, M; Grabellus, F; Schmid, KW; Totsch, M.
Association between apoptotis and CD4(+)/CD8(+) T-lymphocyte ratio in aseptic loosening after total hip replacement.
Int J Biol Sci. 2009; 5(2):182-191
Doi: 10.7150/ijbs.5.182
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- Co-Autor*innen der Med Uni Graz
- Tötsch Martin
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- Abstract:
- Particle-induced osteolysis is a major cause of aseptic loosening after total joint replacement. While the osteolytic cascade initiated by cytokine release from macrophages has been studied extensively, the involvement of T-lymphocytes in this context is controversial and has been addressed by only a few authors. In a former study we detected that the quantity of T-lymphocytes may be influenced by apoptosis in patients with aseptic loosening. In this study we intended to find out more details about the apoptosis-induced shifting of the T-cell number. We focused our interest on the CD4(+) and CD8(+) T-cells and their relative ratio. Caspase-3 cleaved was evaluated immunohistochemically to detect apoptotic T-cells in capsules and interface membranes from patients with aseptic hip implant loosening and a varying degree of caspase-3 cleaved expression in CD4(+) and CD8(+) T-lymphocytes was detected. Moreover, a relationship between the intensity of the apoptotic reactions and the radiological extent of osteolysis was observed. The number of CD4(+) cells was decreased in the presence of strong apoptotic reactions, respectively extensive osteolysis, while CD8(+) cells were affected to a much lower degree. Thus, the CD4(+)/CD8(+) ratio changed from 1.0 in cases with only small areas of periprosthetic osteolysis and minimally intense apoptosis to 0.33 in cases with large areas of osteolysis. This may suggest a causal relationship between the apoptosis-induced shift in the CD4(+)/CD8(+) ratio and the osteolysis respectively aseptic loosening. It is possible that these findings may lead to a new understanding of particle-induced osteolysis.
- Find related publications in this database (using NLM MeSH Indexing)
- Aged -
- Aged, 80 and over -
- Apoptosis -
- Arthroplasty, Replacement, Hip - adverse effects
- CD4-Positive T-Lymphocytes - metabolism
- CD8-Positive T-Lymphocytes - metabolism
- Caspase 3 - metabolism
- Female -
- Hip Prosthesis - adverse effects
- Humans -
- Immunohistochemistry -
- Male -
- Middle Aged -
- Osteolysis - etiology
- Find related publications in this database (Keywords)
- Wear Debris
- Osteolysis
- Apoptosis
- T-Lymphocytes
- Immunohistochemistry