Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Tötsch, M; Brömmelkamp, E; Stücker, A; Fille, M; Gross, R; Wiesner, P; Schmid, KW; Böcker, W; Dockhorn-Dworniczak, B.
Identification of mycobacteria to the species level by automated restriction enzyme fragment length polymorphism analysis.
Virchows Arch. 1995; 427(1):85-89
Doi: 10.1007/BF00203742
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Tötsch Martin
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- Abstract:
- An automated method for the restriction fragment length polymorphism (RFLP) analysis for the differentiation of mycobacteria to the species level is described. After polymerase chain reaction (PCR) amplification of a sequence of the gene encoding the 65-kDa surface antigen common to all mycobacteria the product was investigated by RFLP analysis. For accurate determination of fragment sizes the asymmetrically fluorescein-labelled PCR product was partially digested with restriction site enzymes BstEII and HaeIII. The fragments obtained were analysed electrophoretically using an automated laser fluorescence DNA sequencer. Determination of fragment sizes revealed a deviation of +/- 1 base pair (bp; 0.6%) when compared to expected sizes. The validity of this approach was confirmed by analysing mycobacterial DNA obtained from pure cultures of Mycobacterium (M.) tuberculosis and alcohol-fixed smears as well as paraffin-embedded sputa of patients with culture-proven tuberculosis. Additionally a diagnostic algorithm was established by investigation of cultured M. bovis, M. bovis bacille Calmette-Guérin, M. avium, M. intracellulare and M. fortuitum. The method allows the identification of restriction enzyme sites which are only 40 bp apart. Partial restriction enzyme digestion of asymmetrically fluorescence-labelled PCR products will presumably lead to the discovery of new restriction enzyme sites.
- Find related publications in this database (using NLM MeSH Indexing)
- Algorithms -
- Bacterial Typing Techniques -
- Base Sequence -
- DNA, Bacterial - analysis
- Molecular Sequence Data -
- Mycobacterium - genetics Mycobacterium - isolation and purification
- Polymorphism, Restriction Fragment Length -
- Restriction Mapping -
- Find related publications in this database (Keywords)
- AUTOMATED LASER FLUORESCENCE SEQUENCER
- PARTIAL DIGESTION
- RESTRICTION ENZYME FRAGMENT LENGTH ANALYSIS
- ASYMMETRICALLY LABELED FLUORESCENCE PRIMER
- MYCOBACTERIA