Medizinische Universität Graz - Research portal
Selected Publication:
Tötsch, M; Schmid, KW; Brömmelkamp, E; Stücker, A; Puelacher, C; Sidoroff, G; Mikuz, G; Böcker, W; Dockhorn-Dworniczak, B.
Rapid detection of mycobacterial DNA in clinical samples by multiplex PCR.
Diagn Mol Pathol. 1994; 3(4):260-264
Doi: 10.1097/00019606-199412000-00008
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Tötsch Martin
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Triplex-polymerase chain reaction technique (PCR) was developed for the detection and identification of mycobacterial DNA sequences in uncultured clinical samples. A 123 bp fragment corresponding to a specific Mycobacterium tuberculosis sequence complex, a 383 bp DNA fragment encoding for part of the 65 kD mycobacterial surface antigen, and a 268 bp fragment of the human beta-globin gene to demonstrate the presence of suitable DNA were amplified by triplex PCR. To demonstrate the applicability of this method, 206 alcohol-fixed, paraffin-embedded sputum samples from 47 patients with culture-proven tuberculosis were investigated. Of 206 samples, 157 were PCR positive, resulting in correct diagnosis of tuberculosis in 46 of 47 (97.8%) patients. Furthermore, 165 alcohol-fixed, auramin-stained sputum smears were examined in a blind trial. Triplex PCR revealed tuberculosis in 20 of 21 samples from patients with tuberculosis. In comparison, cultures were positive in 20 of 21 samples, and acid-fast organisms were found by microscopy in 18 of 21 samples. We conclude that triplex PCR is a rapid and sensitive technique for the detection of mycobacterial DNA in uncultured clinical samples and offers equivalent sensitivity (95.2%) and specificity (98.6%) as do culture methods.
- Find related publications in this database (using NLM MeSH Indexing)
- Base Sequence -
- DNA Primers -
- DNA, Bacterial - analysis
- Electrophoresis, Polyacrylamide Gel -
- Gene Amplification -
- Globins - genetics
- Humans -
- Molecular Sequence Data -
- Mycobacterium tuberculosis - genetics
- Paraffin Embedding -
- Polymerase Chain Reaction - methods
- Sputum - chemistry Sputum - microbiology
- Tissue Fixation -
- Tuberculosis - diagnosis
- Find related publications in this database (Keywords)
- MYCOBACTERIA
- MYCOBACTERIUM TUBERCULOSIS
- MULTIPLEX PCR
- UNCULTURED CLINICAL SAMPLES