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Reis, A; Tötsch, M; Shennib, H; Ofner, D; Serrick, C; Jamjoom, A; Chikhani, N; Mikuz, G; Margreiter, R.
Bronchoalveolar lavage: results of sequential, selective techniques in viable murines.
Am J Respir Crit Care Med. 1994; 150(2):547-550 Doi: 10.1164/ajrccm.150.2.8049844
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Co-authors Med Uni Graz: Tötsch Martin
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Abstract:: The purpose of this study was to develop an optimal technique for performing sequential bronchoalveolar lavage (BAL) in a murine animal model. Two general anesthetic regimens and four operative techniques of BAL were tested. Anesthesia by intraperitoneal injection of ketamine hydrochloride (100 mg/kg body wt) resulted in death for four of ten subjects, whereas inhalation of diethyl ether led to death for one of ten subjects. BAL using a balloon catheter under bronchoscopic guidance was comparable with postmortal lavage, tolerated better, and resulted in superior cell retrieval with respect to cell differential (macrophages: 95 +/- 2.3; lymphocytes: 3 +/- 1.2; polymorphonuclear lymphocytes [PNL]: 1.2 +/- 1.4) compared with two other techniques using a bent metal tube/polyethylene tubing combination (macrophages: 19.3 +/- 27.4; lymphocytes: 3.8 +/- 4.3; PNL: 35.5 +/- 35.5) and a bronchoscope/polyethylene tubing combination (macrophages: 11.1 +/- 25.5; lymphocytes: 0.7 +/- 1.0; PNL: 55.8 +/- 41.0). The BAL fluid contained significantly more alveolar macrophages and fewer PNL and epithelial cells (p = 0.0001, p = 0.0025, p = 0.02, respectively). We conclude that the technique using a balloon catheter under bronchoscopic guidance during inhalation of diethyl ether is the procedure of choice and results in a representative sample of BAL.
Find related publications in this database (using NLM MeSH Indexing): Anesthesia -; Animals -; Balloon Dilatation -; Bronchoalveolar Lavage Fluid - cytology; Bronchoscopy -; Cell Count -; Intubation -; Male -; Methods -; Rats -; Rats, Sprague-Dawley -