Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Mittermann, I; Zidarn, M; Silar, M; Markovic-Housley, Z; Aberer, W; Korosec, P; Kosnik, M; Valenta, R.
Recombinant allergen-based IgE testing to distinguish bee and wasp allergy.
J ALLERG CLIN IMMUNOL. 2010; 125(6): 1300-1307.
Doi: 10.1016/j.jaci.2010.03.017
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- Co-Autor*innen der Med Uni Graz
- Aberer Werner
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- Abstract:
- Background: The identification of the disease-causing insect in venom allergy is often difficult. Objective: To establish recombinant allergen-based IgE tests to diagnose bee and yellow jacket wasp allergy. Methods: Sera from patients with bee and/or wasp allergy (n = 43) and patients with pollen allergy with false-positive IgE serology to venom extracts were tested for IgE reactivity in allergen extract-based tests or with purified allergens, including nonglycosylated Escherichia coli expressed recombinant (r) Api m 1, rApi m 2, rVes v 5, and insect cell expressed, glycosylated rApi m 2 as well as 2 natural plant glycoproteins (Phl p 4, bromelain). Results: The patients with venom allergy could be diagnosed with a combination of E coli expressed rApi m 1, rApi m 2, and rVes v 5 whereas patients with pollen allergy remained negative. For a group of 29 patients for whom the sensitizing venom could not be identified with natural allergen extracts, testing with nonglycosylated allergens allowed identification of the sensitizing venom. Recombinant nonglycosylated allergens also allowed definition of the sensitizing venom for those 14 patients who had reacted either with bee or wasp venom extracts. By IgE inhibition studies, it is shown that glycosylated Api m 2 contains carbohydrate epitopes that cross-react with natural Api m 1, Ves v 2, natural Phl p 4, and bromelain, thus identifying cross-reactive structures responsible for serologic false-positive test results or double-positivity to bee and wasp extracts. Conclusion: Nonglycosylated recombinant bee and wasp venom allergens allow the identification of patients with bee and wasp allergy and should facilitate accurate prescription of venom immunotherapy. (J Allergy Clin Immunol 2010;125:1300-7.)
- Find related publications in this database (using NLM MeSH Indexing)
- Allergens - genetics
- Animals -
- Antigens, Plant -
- Bees -
- Cells, Cultured -
- Cloning, Molecular -
- Diagnosis, Differential -
- False Positive Reactions -
- Female -
- Humans -
- Hypersensitivity, Immediate - complications
- Immunoglobulin E - immunology
- Insect Proteins - genetics
- Male -
- Phospholipases A - genetics
- Recombinant Proteins - genetics
- Rhinitis, Allergic, Seasonal - complications
- Serology -
- Venoms -
- Wasps -
- Find related publications in this database (Keywords)
- Venom allergy
- recombinant allergen
- in vitro diagnosis
- glycosylation