Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Macher, S; Sipurzynski-Budraß, S; Rosskopf, K; Rohde, E; Griesbacher, A; Groselj-Strele, A; Lanzer, G; Schallmoser, K.
Function and activation state of platelets in vitro depend on apheresis modality.
Vox Sang. 2010; 99(4): 332-340.
Doi: 10.1111/j.1423-0410.2010.01353.x
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Lanzer Gerhard
- Macher Susanne
- Schallmoser Katharina
- Co-Autor*innen der Med Uni Graz
- Griesbacher Antonia
- Groselj-Strele Andrea
- Rohde Eva
- Rosskopf Konrad
- Sipurzynski Sabine
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- Abstract:
- Background and Objectives In multicomponent collection, various blood components are prepared during one apheresis process. The aim of this prospective crossover study was to compare the function, metabolic parameters and activation state of fresh and stored platelets (PLTs) collected by two different cell separators. Materials and Methods Twenty-four donors underwent apheresis on each of two cell separators (Fenwal Amicus (R) and CaridianBCT Trima Accel (R)) with an interval of at least 2 months between donations. Per donation, one double dose of PLT concentrate (PC) and one unit of packed red-blood-cells were collected. In total, 48 single unit PCs were tested for pH, glucose, bicarbonate, lactate, potassium and LDH concentration during 7 days of storage. PLT function was analysed by aggregometry, rotation thrombelastometry and hypotonic shock response. The PLT surface expression of P-selectin (CD62P) and LAMP-3 (CD63) was estimated by flow cytometry. Results During storage, metabolic parameters were well maintained in both groups, but levels of glucose and pH were significantly lower, while lactate and LDH were significantly higher in Amicus (R)-PCs. Amicus (R)-derived PLTs were significantly more activated as evidenced by higher CD62P and CD63 expression. In parallel, the in vitro function of Amicus (R)-PLTs was significantly reduced compared to Trima (R)-PLTs. Conclusion In multicomponent apheresis, standardized PLT collection is effective and well tolerated. The higher activation of Amicus (R)-derived PLTs may be because of the divergent centrifugation modalities during collection. Possible consequences for the clinical outcome of thrombocytopenic patients will be evaluated in further trials.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Antigens, CD - biosynthesis
- Antigens, CD63 -
- Blood Component Removal -
- Blood Donors -
- Blood Platelets - cytology
- Cross-Over Studies -
- Female -
- Gene Expression Regulation -
- Humans -
- Male -
- Middle Aged -
- Osmotic Pressure -
- P-Selectin - biosynthesis
- Platelet Activation -
- Platelet Membrane Glycoproteins - biosynthesis
- Prospective Studies -
- Refrigeration -
- Thrombelastography -
- Time Factors -
- Find related publications in this database (Keywords)
- multicomponent apheresis
- Fenwal Amicus (R)
- CaridianBCT Trima Accel (R)
- platelet function
- platelet activation