Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Bundschu, K; Gattenlohner, S; Knobeloch, KP; Walter, U; Schuh, K.
Tissue-specific Spred-2 promoter activity characterized by a gene trap approach.
Gene Expr Patterns. 2006; 6(3):247-255
Doi: 10.1016/j.modgep.2005.08.003
Web of Science
PubMed
FullText
FullText_MUG
- Co-authors Med Uni Graz
- Gattenlöhner Stefan
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- Abstract:
- Spreds (Sprouty-related proteins with an Ena/Vasodilator-stimulated phosphoprotein homology-1 domain) are a new protein family inhibiting the mitogen-activated protein kinase (MAPK) signaling pathway. Different RNA and protein studies already revealed an almost ubiquitous Spred-2 expression pattern. But until now, only few data were available on the in situ Spred-2 promoter activity. Here, we show a detailed in situ analysis of a mouse strain with a trapped Spred-2 gene, bringing a beta-galactosidase and neomycin fusion gene (beta-geo) under the control of the endogenous Spred-2 promoter. This allowed us to monitor Spred-2 promoter activity in practically every organ and their corresponding sub-compartments. X-Gal staining of newborn and adult mice revealed a nearly congruent Spred-2 promoter activity pattern. Our detailed data provide information for further studies of the still enigmatic physiological functions of Spred-2 in various organs by identifying the tissues with strong Spred-2 promoter activity.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Animals, Newborn -
- Cell Line -
- Crosses, Genetic -
- DNA, Complementary - genetics
- Exons -
- Female -
- Galactosides - metabolism
- Gene Expression Regulation, Developmental -
- Gene Targeting -
- Genes, Reporter -
- Genetic Vectors -
- Immunohistochemistry -
- Indoles - metabolism
- Introns -
- Male -
- Mice -
- Mice, Inbred C57BL -
- Mice, Knockout -
- Mitogen-Activated Protein Kinases - antagonists and inhibitors
- Muscle, Smooth - metabolism
- Promoter Regions, Genetic -
- Protein Structure, Tertiary -
- Repressor Proteins - chemistry Repressor Proteins - genetics Repressor Proteins - metabolism
- Stem Cells - cytology
- Find related publications in this database (Keywords)
- Spred-2
- gene trap
- promoter activity
- X-Gal staining