Selected Publication:

SHR Neuro Cancer Cardio Lipid Metab Microb

Friedl, G; Schmidt, H; Rehak, I; Kostner, G; Schauenstein, K; Windhager, R.
Undifferentiated human mesenchymal stem cells (hMSCs) are highly sensitive to mechanical strain: transcriptionally controlled early osteo-chondrogenic response in vitro.
Osteoarthritis Cartilage. 2007; 15(11): 1293-1300. Doi: 10.1016/j.joca.2007.04.002 [OPEN ACCESS]
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Leading authors Med Uni Graz

Friedl Gerald

Windhager Reinhard

Co-authors Med Uni Graz

Kostner Gerhard

Schmidt Helena

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Abstract:

OBJECTIVE: Physical cues play a crucial role in skeletogenesis and osteochondral regeneration. Although human mesenchymal stem cells (hMSCs) offer considerable therapeutic potential, little is known about the molecular mechanisms that control their differentiation. We hypothesized that mechanical strain might be an inherent stimulus for chondrogenic and/or osteogenic differentiation in undifferentiated hMSCs, where c-Fos (FOS) might play a major role in mechanotransduction. METHOD: hMSCs from 10 donors were intermittently stimulated by cyclic tensile strain (CTS) at 3000 mustrain for a period of 3 days. Differential gene expression of strained and unstrained hMSCs was analysed by real-time RT-PCR for several marker genes, including the transcription factors FOS, RUNX2, SOX9, and others. Additionally, alkaline phosphatase activity (ALP) was determined kinetically. RESULTS: The application of CTS significantly stimulated the expression levels of the early chondrogenic and osteogenic marker genes (SOX9, LUM, DCN; RUNX2, SPARC, SPP1, ALPL); this was accompanied by stimulation of ALP activity (+38%+/-12 standard error of mean, P<0.05). Matrix analysis revealed that the osteo-chondrogenic response followed a coordinated expression pattern, in which FOS was attributed to early osteogenic but not chondrogenic differentiation. CONCLUSION: Undifferentiated hMSCs are highly sensitive to mechanical strain with a transcriptionally controlled osteo-chondrogenic differentiation response in vitro.

Find related publications in this database (using NLM MeSH Indexing)

Adult -

Aged -

Aged, 80 and over -

Alkaline Phosphatase - analysis

Biological Markers - metabolism

Bone Marrow Cells - physiology

Cells, Cultured - physiology

Chondrogenesis - genetics

Female - genetics

Humans - genetics

Male - genetics

Mesenchymal Stem Cells - enzymology

Middle Aged - enzymology

Osteogenesis - genetics

Reverse Transcriptase Polymerase Chain Reaction - genetics

Stress, Mechanical - genetics

Stromal Cells - physiology

Transcription Factors - genetics

Find related publications in this database (Keywords)

bone marrow stromal cells

osteogenesis

chondrogenesis

endochondral ossification

c-Fos

integrin receptors

mechanical strain

donor-to-donor variance

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