Selected Publication:

SHR Neuro Cancer Cardio Lipid Metab Microb

Lindegaard, ML; Olivecrona, G; Christoffersen, C; Kratky, D; Hannibal, J; Petersen, BL; Zechner, R; Damm, P; Nielsen, LB.
Endothelial and lipoprotein lipases in human and mouse placenta.
J LIPID RES. 2005; 46: 2339-2346. Doi: 10.1194/jlr.M500277-JLR200 [OPEN ACCESS]
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Co-authors Med Uni Graz

Kratky Dagmar

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Abstract:

Placenta expresses various lipase activities. However, a detailed characterization of the involved genes and proteins is lacking. In this study, we compared the expression of endothelial lipase (EL) and LPL in human term placenta. When placental protein extracts were separated by heparin-Sepharose affinity chromatography, the EL protein eluted as a single peak without detectable phospholipid or triglyceride (TG) lipase activity. The major portion of LPL protein eluted slightly after EL. This peak also had no lipase activity and most likely contained monomeric LPL. Fractions eluting at a higher NaCl concentration contained small amounts of LPL protein (most likely dimeric LPL) and had substantial TG lipase activity. In situ hybridization studies showed EL mRNA expression in syncytiotrophoblasts and endothelial cells and LPL mRNA in syncytiotrophoblasts. In contrast, immunohistochemistry showed EL and LPL protein associated with both cell types. In mouse placentas, lack of LPL expression resulted in increased EL mRNA expression. These results suggest that the cellular expression of EL and LPL in human placenta is different. Nevertheless, the two lipases might have overlapping functions in the mouse placenta. Our data also suggest that the major portions of both proteins are stored in an inactive form in human term placenta.

Find related publications in this database (using NLM MeSH Indexing)

Animals -

Biopsy -

Blotting, Western -

Chromatography, Affinity -

Dimerization -

Endothelium - enzymology

Heparin - chemistry

Humans - chemistry

Immunohistochemistry - chemistry

In Situ Hybridization - chemistry

Lipoprotein Lipase - chemistry

Mice - chemistry

Mice, Transgenic - chemistry

Phospholipids - chemistry

Placenta - enzymology

RNA, Messenger - metabolism

Research Support, Non-U.S. Gov't - metabolism

Sepharose - chemistry

Sodium Chloride - pharmacology

Species Specificity - pharmacology

Triglycerides - chemistry

Trophoblasts - metabolism

Find related publications in this database (Keywords)

lipid transport

in situ hybridization

immunohistochemistry

lipase activity

lipoprotein lipase deficiency

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