Selected Publication:

SHR Neuro Cancer Cardio Lipid Metab Microb

Rossmann, C; Darko, A; Kager, G; Ledinski, G; Wonisch, W; Wagner, T; Hallström, S; Reibnegger, G; Paar, M; Cvirn, G.
Natural Polyamine Spermidine Inhibits the In Vitro Oxidation of LDL.
Molecules. 2025; 30(4): 955 Doi: 10.3390/molecules30040955 [OPEN ACCESS]
Web of Science PubMed FullText FullText_MUG

 

Leading authors Med Uni Graz

Cvirn Gerhard

Rossmann Christine Renate

Co-authors Med Uni Graz

Hallström Seth

Kager Gerd

Ledinski Gerhard

Paar Margret

Reibnegger Gilbert

Wagner Thomas

Wonisch Willibald

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Abstract:

Spermidine is a natural autophagy-inducer and anti-aging compound. Herein, we investigated a potential autophagy-independent mechanism of spermidine, namely its capability to directly impede LDL oxidation, an early step in atherogenesis. In our in vitro-model, LDL oxidation was induced by the addition of CuCl₂ in the presence of increasing concentrations of spermidine, and the degree of oxidation of the lipid, as well as of the protein part of LDL, was measured. We found that spermidine concentration-dependently inhibited the production of lipid hydroperoxides, malondialdehyde, and oxidation-specific immune epitopes in the LDL particle, associated with decreased relative electrophoretic mobilities, respectively. For example, the LPO content was significantly lower when LDL was oxidized in the presence of 500 µg/mL spermidine (26.9 ± 1.6 nmol/mg LDL) than in the absence of spermidine (180.6 ± 7.7 nmol/mg LDL, p < 0.0001). When oxLDL was obtained under increasing spermidine concentrations, its cytotoxicity in EA.hy926 cells concentration-dependently decreased. Quantum chemical calculations show that the reaction between spermidine and hydroxyl radicals is exergonic. We conclude that spermidine is a direct inhibitor of LDL oxidation due to its capability to scavenge hydroxyl radicals. Thus, spermidine supplementation might be a suitable tool to impede atherogenesis and associated (cardio)vascular diseases. Further prospective clinical studies are needed to evaluate the potential atheroprotective/health-promoting effects of spermidine-rich diets.

Find related publications in this database (using NLM MeSH Indexing)

Spermidine - pharmacology, chemistry, metabolism

Lipoproteins, LDL - metabolism

Humans - administration & dosage

Oxidation-Reduction - drug effects

Malondialdehyde - metabolism

Lipid Peroxidation - drug effects

Cell Line - administration & dosage

Find related publications in this database (Keywords)

antioxidants

atherogenesis

copper ions

endothelial damage

lipid oxidation

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