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SHR Neuro Cancer Cardio Lipid Metab Microb

Rotta, G; Achille, V; Bornheimer, SJ; Duenas, M; Fernandez, M; Gatti, A; Giron, SH; Rio, IM; Massanella, M; Jiménez, DJ; Monserrat, J; Pfeifer, V; Pichler, J; Prietl, B; Sourij, H; Uras, CRM; Vlah, S; Fenoglio, D.
Protocol for flow cytometry immunophenotyping of human antigen-specific T cells by activation-induced marker and Th1 cytokine detection.
STAR Protoc. 2025; 6(1):103343 Doi: 10.1016/j.xpro.2024.103343 [OPEN ACCESS]
Web of Science PubMed FullText FullText_MUG

 

Co-authors Med Uni Graz

Pfeifer Verena

Prietl Barbara

Sourij Harald

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Abstract:

Flow cytometry characterization of antigen-specific polyfunctional T cells is a valuable tool to study adaptive immunity. Here, we present a protocol for flow cytometry immunophenotyping of human antigen-specific T cells by activation-induced marker (AIM) and Th1 cytokine detection. We describe steps for preparing peripheral blood mononuclear cells (PBMCs) for stimulation followed by washing and staining PBMCs for flow cytometry. We then detail procedures for acquisition and analysis. This protocol has potential applications in the field of vaccine immunology and immuno-oncology. For complete details on the use and execution of this protocol, please refer to Altosole et al.¹.

Find related publications in this database (using NLM MeSH Indexing)

Humans - administration & dosage

Flow Cytometry - methods

Immunophenotyping - methods

Cytokines - analysis, metabolism, immunology

Th1 Cells - immunology, metabolism

Leukocytes, Mononuclear - immunology, cytology, metabolism

Lymphocyte Activation - immunology

Biomarkers - analysis

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