Selected Publication:

SHR Neuro Cancer Cardio Lipid Metab Microb

Vidakovic, I; Kornmueller, K; Fiedler, D; Khinast, J; Fröhlich, E; Leitinger, G; Horn, C; Quehenberger, J; Spadiut, O; Prassl, R.
Archaeosomes for Oral Drug Delivery: From Continuous Microfluidics Production to Powdered Formulations.
Pharmaceutics. 2024; 16(6): 694 Doi: 10.3390/pharmaceutics16060694 [OPEN ACCESS]
Web of Science PubMed FullText FullText_MUG

 

Leading authors Med Uni Graz

Kornmüller Karin

Prassl Ruth

Vidakovic Ivan

Co-authors Med Uni Graz

Fröhlich Eleonore

Leitinger Gerd

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Abstract:

Archaeosomes were manufactured from natural archaeal lipids by a microfluidics-assisted single-step production method utilizing a mixture of di- and tetraether lipids extracted from Sulfolobus acidocaldarius. The primary aim of this study was to investigate the exceptional stability of archaeosomes as potential carriers for oral drug delivery, with a focus on powdered formulations. The archaeosomes were negatively charged with a size of approximately 100 nm and a low polydispersity index. To assess their suitability for oral delivery, the archaeosomes were loaded with two model drugs: calcein, a fluorescent compound, and insulin, a peptide hormone. The archaeosomes demonstrated high stability in simulated intestinal fluids, with only 5% of the encapsulated compounds being released after 24 h, regardless of the presence of degrading enzymes or extremely acidic pH values such as those found in the stomach. In a co-culture cell model system mimicking the intestinal barrier, the archaeosomes showed strong adhesion to the cell membranes, facilitating a slow release of contents. The archaeosomes were loaded with insulin in a single-step procedure achieving an encapsulation efficiency of approximately 35%. These particles have been exposed to extreme manufacturing temperatures during freeze-drying and spray-drying processes, demonstrating remarkable resilience under these harsh conditions. The fabrication of stable dry powder formulations of archaeosomes represents a promising advancement toward the development of solid dosage forms for oral delivery of biological drugs.

Find related publications in this database (Keywords)

archaeosomes

archaeal lipids

oral drug delivery

insulin

dry powder formulation

solid dosage form

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