Selected Publication:

SHR Neuro Cancer Cardio Lipid Metab Microb

Diener, C; Hoge, ACH; Kearney, SM; Kusebauch, U; Patwardhan, S; Moritz, RL; Erdman, SE; Gibbons, SM.
Non-responder phenotype reveals apparent microbiome-wide antibiotic tolerance in the murine gut.
Commun Biol. 2021; 4(1): 316 Doi: 10.1038/s42003-021-01841-8 [OPEN ACCESS]
Web of Science PubMed FullText FullText_MUG

 

Leading authors Med Uni Graz

Diener Christian

Altmetrics:

Dimensions Citations:

Plum Analytics:

Scite (citation analytics):

Abstract:

Broad spectrum antibiotics cause both transient and lasting damage to the ecology of the gut microbiome. Antibiotic-induced loss of gut bacterial diversity has been linked to susceptibility to enteric infections. Prior work on subtherapeutic antibiotic treatment in humans and non-human animals has suggested that entire gut communities may exhibit tolerance phenotypes. In this study, we validate the existence of these community tolerance phenotypes in the murine gut and explore how antibiotic treatment duration or a diet enriched in antimicrobial phytochemicals might influence the frequency of this phenotype. Almost a third of mice exhibited whole-community tolerance to a high dose of the β-lactam antibiotic cefoperazone, independent of antibiotic treatment duration or dietary phytochemical amendment. We observed few compositional differences between non-responder microbiota during antibiotic treatment and the untreated control microbiota. However, gene expression was vastly different between non-responder microbiota and controls during treatment, with non-responder communities showing an upregulation of antimicrobial tolerance genes, like efflux transporters, and a down-regulation of central metabolism. Future work should focus on what specific host- or microbiome-associated factors are responsible for tipping communities between responder and non-responder phenotypes so that we might learn to harness this phenomenon to protect our microbiota from routine antibiotic treatment.

Find related publications in this database (using NLM MeSH Indexing)

Animals - administration & dosage

Female - administration & dosage

Animal Feed - administration & dosage

Anti-Bacterial Agents - pharmacology

Bacteria - drug effects, genetics, growth & development

Cefoperazone - pharmacology

Dysbiosis - administration & dosage

Feces - microbiology

Gastrointestinal Microbiome - drug effects

Genotype - administration & dosage

Intestines - microbiology

Mice, Inbred C57BL - administration & dosage

Phenotype - administration & dosage

Seaweed - administration & dosage

Time Factors - administration & dosage

Drug Resistance, Bacterial - administration & dosage

Mice - administration & dosage

© Med Uni Graz • Imprint